DSPE-PEG-COOH用于制备DSPE-PEG-DOX的相关文献（含合成路线）

专著名: Peptide Engraftment on PEGylated Nanoliposomes for Bone Specific Delivery of PTH (1-34) in Osteoporosis我们：Taewon Hwang 1, Hee Dong Han, Chung Kil Song, Hasoo Seong, Jung Hyun Kim, Xiaoyuan Chen, Byung Cheol Shin绪论：治癌口服药的肉瘤特女性朋友递送是口服药设计方式中 中要面临的大部分试炼之四。在仅仅论述中，各位可以按照将新制成的DSPE-PEG2000-DOX（DPD）对于脂质组成成分掺杂脂质体中光催化原理了阿霉素（DOX）偶联脂质体（DCL），并测试测试了其身体内部抗肉瘤催化活化。可以按照酰胺键将DOX偶联到DSPE-PEG2000-COOH上制成了DPD，并可以按照（1）H-NMR数据具体分析确定了所有DPD的催化的结构。可以按照塑料薄膜浇铸水范法光催化原理了脂质体面积为130nm的DCL。普鲁士蓝染色癌细胞膜术数据具体分析核实，DCL的癌细胞膜摄取量清晰要高于传统艺术的脂质体。DCL对小鼠B16F10黑灰着色剂瘤荷瘤小鼠的抗肉瘤催化活化是因为，DCL比传统艺术的脂质体更有效率地抑止肉瘤生长发育，这有机会是考虑到DOX介导的内吞方式中 。 

实验使用方法:

Synthesis ofConjugate (SDSSD-DSPE)A lipid-PEG-peptide combination that may subsequently be added to liposomes forbone targeting was developed, SDSSD was conjugated to DSPE-PEG2000-C00H usingcarbodiimide chemistry, based on the previously described method by Wang et al. withmodi fications [32. Briefly, DSPE-PEG2000-COOH was dissolved in 0.1 M MES bufferat pH 6.0 and stirred at 2-8 °C for 10 min. Then, EDC.HCl and NHS (20 equivalentseach) were added to the reaction mixture and stirred for 60 min at 2-8 °C. A 7.4 pHadjustment was made to the reaction mixture, Finally, SDSSD, previously dissolved inMilli-Q water, was added to the reaction mixture and stirred overnight. The resultantmixture was dialyzed using a 2KD dialysis membrane for 48 h to remove unconjugatedSDSSD. Purifield SDSSD-DSPE (conjugate) was freeze-dried and characterized by'H-NMRMaldi-TOF, and a Scanning Electron Microscope (SEM) followed by Energy Dispersive。

 

  

反应结构路线

 

重要检测:

文献核磁

 

主要實驗最终解释Synthesis of DSPE-PEG2000-DOX (DPD)Covalent conjugation of DOX to DSPE.PEG2000-COOH was confirmed by HNMR (500 MHz, Auto Sampler-HRMAS.FT-NMR, Bruker, Switzerland) analysis ofDPD. 'HNMR spectra were obtained forcomb polymer solutions in deuteratedchloroform. As shown in a Figure 3, theproton peaks of DOX (1,2 and 3)andDSPE-PEG2000-C00H(4 and 5)wereobserved at 7.4~8.2 ppm and 1.2~3.7ppm, respectively.

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制造厂:郑州pg电子娱乐游戏app 动物科技有限的总部有限的总部

应用领域:科研项目

情形：气体/粉状/饱和溶液

主产地:昆明

贴心警醒:未经许可科技，不用在人身体工作!

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