DSPE-PEG-COOH用于制备DSPE-PEG-DOX的相关文献（含合成路线）

论文名: Peptide Engraftment on PEGylated Nanoliposomes for Bone Specific Delivery of PTH (1-34) in Osteoporosis小说作品：Taewon Hwang 1, Hee Dong Han, Chung Kil Song, Hasoo Seong, Jung Hyun Kim, Xiaoyuan Chen, Byung Cheol Shin引言：*癌类药物治疗的肉瘤特异形递送是类药物治疗联合开发操作步骤中遭受的关键挑站之四。在此项探讨中，企业顺利凭借将新组成的DSPE-PEG2000-DOX（DPD）做为脂质成份添加脂质体中配制了阿霉素（DOX）偶联脂质体（DCL），并检验了其休内*肉瘤亲水性。顺利凭借酰胺键将DOX偶联到DSPE-PEG2000-COOH上组成了DPD，并顺利凭借（1）H-NMR分折根本了应纳税所得额DPD的物理化学型式。顺利凭借聚酯薄膜浇铸水违法配制了脂质体长度为130nm的DCL。流式的生殖癌细胞术分折断定，DCL的生殖癌细胞摄入强烈远远超出常用脂质体。DCL对小鼠B16F10一样素瘤荷瘤小鼠的*肉瘤亲水性表面，DCL比常用脂质体更可行地可抑制肉瘤植物生长，这概率是根据DOX介导的内吞操作步骤。 

实验使用方法:

Synthesis ofConjugate (SDSSD-DSPE)A lipid-PEG-peptide combination that may subsequently be added to liposomes forbone targeting was developed, SDSSD was conjugated to DSPE-PEG2000-C00H usingcarbodiimide chemistry, based on the previously described method by Wang et al. withmodi fications [32. Briefly, DSPE-PEG2000-COOH was dissolved in 0.1 M MES bufferat pH 6.0 and stirred at 2-8 °C for 10 min. Then, EDC.HCl and NHS (20 equivalentseach) were added to the reaction mixture and stirred for 60 min at 2-8 °C. A 7.4 pHadjustment was made to the reaction mixture, Finally, SDSSD, previously dissolved inMilli-Q water, was added to the reaction mixture and stirred overnight. The resultantmixture was dialyzed using a 2KD dialysis membrane for 48 h to remove unconjugatedSDSSD. Purifield SDSSD-DSPE (conjugate) was freeze-dried and characterized by'H-NMRMaldi-TOF, and a Scanning Electron Microscope (SEM) followed by Energy Dispersive。

 

  

反应结构路线

 

重要检测:

文献核磁

 

主要實驗数据详细说明Synthesis of DSPE-PEG2000-DOX (DPD)Covalent conjugation of DOX to DSPE.PEG2000-COOH was confirmed by HNMR (500 MHz, Auto Sampler-HRMAS.FT-NMR, Bruker, Switzerland) analysis ofDPD. 'HNMR spectra were obtained forcomb polymer solutions in deuteratedchloroform. As shown in a Figure 3, theproton peaks of DOX (1,2 and 3)andDSPE-PEG2000-C00H(4 and 5)wereobserved at 7.4~8.2 ppm and 1.2~3.7ppm, respectively.

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工厂:杭州pg电子娱乐游戏app 微生物技术现有有限公司英文

使用:科研工作

心态：粉尘状/粉尘/硫酸铜溶液

產地:浙江

浪漫小编提醒:仅作教学科研，是不能用做身休试验!

有关系车辆：

‌DSPE-PEG-α-Syn‌（α-突触核淀粉酶彻底清除肽）‌‌DSPE-PEG-TrkB‌（周围神经莒养因素感觉冲动肽）‌‌DSPE-PEG-GABA‌（γ-氨基丁酸蛋白激酶上下调整肽）‌‌DSPE-PEG-5-HT‌（5-羟色胺蛋白激酶靶向药物）‌‌DSPE-PEG-AMPA‌（谷氨酸多巴胺受体的调节肽）‌‌DSPE-PEG-BMP-2‌（骨价值形式情况淀粉酶模似肽）‌‌DSPE-PEG-VEGF‌（血管壁内皮繁殖因素养成肽）‌‌DSPE-PEG-FGF2‌（成纤维棉上皮细胞衍生要素靶向药物）‌‌DSPE-PEG-EPO‌（促红肿瘤细胞添加素模拟机肽）‌