DSPE-PEG-NHS介导的CpG递送系统化设计与免疫系统化学活化判断外部链接：//link.springer.com/article/10.1007/s12257-020-0366-1我们：Jiwon Yang, Eun Seo Choi, Gayeon You & Hyejung Mok 引言：综上所述CpG寡脱氧核苷酸（CpG）具强 的抗体检测抗体激发使用，的搭建CpG质粒是达成提高率肿瘤抗体检测抗体*的先决标准。本研究分析将1,2-二硬脂酰-sn-甘油-3-磷酸乙酸乙酯胺-N-[羟基蜜腊酰亚胺（聚乙二醇）] (DSPE-PEG-NHS) 与聚氯乙烯亚胺 (PEI) 偶联，的搭建出的PEI-PEG-DSPE偶联物，能作为业物相匹配性的提高率CpG质粒。自己的搭建了这几种PEIPEG-DSPE偶联物，每一种偶联物的PEI碳原子量差异，DSPEPEG的修饰语数量也差异，均主要表现出为显著性较低的神经元致癌性。详细所说，与借助先天PEI递送CpG相比较，以摩尔比0.1递送PEI (25 kDa)-PEG-DSPE和DSPE-PEG-NHS/（PEI的胺基）可从而导致RAW264.7神经元对CpG的降解更好，这或者是可能有着疏水性聚氨酯脂质一部分。不仅而且，PEI-PEG-DSPE/CpG和好物可引诱RAW264.7神经元为显著性合成神经元因素（TNF-α），其使用与PEI/CpG和好物一样。往往，PEI-PEG-DSPE偶联物能作为业物相匹配性的提高率质粒，将抗体检测抗体激发剂CpG递送回巨噬神经元。译文翻译：Considering the potent immune stimulation by CpG oligodeoxynucleotides (CpGs), the development of CpG carriers is a prerequisite for efficient cancer immunotherapy. In this study, we conjugated 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[hydroxyl succinimidyl (polyethylene glycol)] (DSPE-PEG-NHS) with polyethylenimine (PEI) to develop a PEI-PEG-DSPE conjugate that can serve as a biocompatible and efficient CpG carrier. Five types of PEIPEG-DSPE conjugates were developed, each with different molecular weights of PEI and different degrees of DSPEPEG modification, and all exhibited significantly lower cytotoxicity. In particular, compared to CpG delivery via natural PEI, delivery with PEI (25 kDa)-PEG-DSPE and DSPE-PEG-NHS/(amine groups of PEI) at a molar ratio of 0.1 resulted in a higher uptake of CpGs into RAW264.7 cells, probably because of the presence of a hydrophobic lipid moiety. In addition, PEI-PEG-DSPE/CpG complexes triggered significant cytokine secretion (TNF-α) from RAW264.7 cells, comparable to that triggered by PEI/CpG complexes. Thus, PEI-PEG-DSPE conjugates could serve as biocompatible and efficient carriers of the immune stimulator CpG to the macrophages.

宝鸡pg电子娱乐游戏app 生物技术提供数据相关的产品的：DSPE-PEG-VIP(二硬脂酰基磷脂酰酒精胺-聚乙二醇-*血管壁几丁质酶肠肽)DSPE-PEG-GE11(二硬脂酰基磷脂酰甲醇胺-聚乙二醇-*细胞核皮下组织的成长细胞EGFR靶点肽)DSPE-PEG-PTP(二硬脂酰基磷脂酰无水乙醇胺-聚乙二醇-胰腺癌靶向药物肽)DSPE-PEG-FSHB(二硬脂酰基磷脂酰乙酸乙酯胺-聚乙二醇-暖巢癌靶向治疗肽)DSPE-PEG-R6H4（二硬脂酰基磷脂酰甲醇胺-聚乙二醇-PH反映性细胞系穿膜肽)DSPE-PEG-SEARYL-R8（二硬脂酰基磷脂酰乙酸乙酯胺-聚乙二醇-PH为了响应性生殖细胞穿膜肽)DSPE-PEG-SEARYL-PF6（二硬脂酰基磷脂酰酒精胺-聚乙二醇-PH出错性細胞穿膜肽)DSPE-PEG-TH（二硬脂酰基磷脂酰工业乙醇胺-聚乙二醇-PH回复性人体细胞穿膜肽)DSPE-PEG-OTC（二硬脂酰基磷脂酰甲醇胺-聚乙二醇-发展抑索感觉靶向药物肽)上短文项目源于当下杂志或专著，深表歉意商标侵权请关联咱们删除图片！