文献：Design of pH-sensitive methotrexate prodrug-targeted curcumin nanoparticles for efficient dual-drug delivery and combination cancer therapy

医学文献环节：//www.tandfonline.com/doi/full/10.2147/IJN.S152312#d1e205小说作者：Jiajiang Xie,Zhongxiong Fan,Yang Li,Yinying Zhang,Fei Yu,Guanghao Su

论文摘要：

To investigate the intracellular drug/nanocarrier delivery of MTX-Imine-M-CUR, Cy5.5-labeled DSPE-PEG was synthesized for use as a near-infrared fluorescent probe (DSPE-PEG-Cy5.5 was conjugated between DSPE-PEG-NH2 and Cy5.5 NHS ester via amide linkage). HeLa cells were seeded into 24-well plates at a density of 1.0×104 cells per well. After culturing for 24 h, the cells were treated with MTX-Imine-MCy5.5-CUR at 37°C for predetermined incubation time periods. Afterward, the cells were washed three times with PBS and fixed with 4% (v/v) paraformaldehyde for 15 min. Finally, the cells were washed by cold PBS twice, stained with DAPI for 10 min, and observed using a Leica TCS SP5 CLSM.

是为了研究方案MTX-Imine-M-CUR的細胞内口服药物/nm质粒载体递送，分解成了Cy5.5标示的DSPE-PEG对于近红外荧光探头（DSPE-PEG-Cy5.5进行酰胺键搭配在DSPE-PEG-NH2和Cy5.5 NHS酯相互之间）。HeLa神经受损神经组织细胞以每孔1.0×104个神经受损神经组织细胞的体积密度打疫苗到24孔板中。锻炼24小的时候后，在37°C下要MTX-Imine-MCy5.5-CUR除理神经受损神经组织细胞既定的孵育时刻。随后，用PBS洗衣机清洗神经受损神经组织细胞四次，用4%（v/v）多聚零甲醛无污染特定1两小时。结果，用冷PBS清洗上皮细胞三次，用DAPI着色1015分钟，并运用Leica TCS SP5 CLSM分析。有关于比较适合车辆：Cy7-PEG-DPPEDSPE-PEG-Cy7DSPE-PEG-cRGDDPPE-PEG-cRGDDMPE-PEG-cRGDDOPE-PEG-cRGDDPPE-PEG-RGDDMPE-PEG-RGDDOPE-PEG-RGD4-Arm PEG-DSPEC12-PEG-COOH上面论文网站内容源各大中文核心期刊或资料，告之侵犯著作权请练习他们卸载！