文献：Design of pH-sensitive methotrexate prodrug-targeted curcumin nanoparticles for efficient dual-drug delivery and combination cancer therapy

医学文献微信链接：//www.tandfonline.com/doi/full/10.2147/IJN.S152312#d1e205小说家：Jiajiang Xie,Zhongxiong Fan,Yang Li,Yinying Zhang,Fei Yu,Guanghao Su

结语：

To investigate the intracellular drug/nanocarrier delivery of MTX-Imine-M-CUR, Cy5.5-labeled DSPE-PEG was synthesized for use as a near-infrared fluorescent probe (DSPE-PEG-Cy5.5 was conjugated between DSPE-PEG-NH2 and Cy5.5 NHS ester via amide linkage). HeLa cells were seeded into 24-well plates at a density of 1.0×104 cells per well. After culturing for 24 h, the cells were treated with MTX-Imine-MCy5.5-CUR at 37°C for predetermined incubation time periods. Afterward, the cells were washed three times with PBS and fixed with 4% (v/v) paraformaldehyde for 15 min. Finally, the cells were washed by cold PBS twice, stained with DAPI for 10 min, and observed using a Leica TCS SP5 CLSM.

方便研究方案MTX-Imine-M-CUR的细胞核内药物剂量/納米形式递送，制成了Cy5.5符号的DSPE-PEG身为近红外荧光检测器（DSPE-PEG-Cy5.5在酰胺键融合在DSPE-PEG-NH2和Cy5.5 NHS酯之前）。HeLa组织以每孔1.0×104个组织的密度计算公式预防接种到24孔板中。培植24小的时候后，在37°C下使用MTX-Imine-MCy5.5-CUR操作组织订购的孵育耗时。随后，用PBS洗衣机清洗组织三遍，用4%（v/v）多聚甲醛污染不变1五一分钟。结果，用冷PBS洗滌内部两回，用DAPI染料1030分钟，并操作Leica TCS SP5 CLSM考察。关于安利软件：Cy7-PEG-DPPEDSPE-PEG-Cy7DSPE-PEG-cRGDDPPE-PEG-cRGDDMPE-PEG-cRGDDOPE-PEG-cRGDDPPE-PEG-RGDDMPE-PEG-RGDDOPE-PEG-RGD4-Arm PEG-DSPEC12-PEG-COOH之上论文信息内容从何而来各项论文期刊或文献综述，见谅版权侵权请建立联系各位卸载！