文献：DOPC-Detergent Conjugates: Fusogenic Carriers for Improved In Vitro and In Vivo Gene Delivery

原作者：Philippe Pierrat, Anne Casset, Dimitri Kereselidze, Marie Lux, Françoise Pons, Luc Lebeau医学文献网页链接： 文献综述：Phospholipid-detergent conjugates are proposed as fusogenic carriers for gene delivery. Eleven compounds are prepared and their properties are investigated. The ability of the conjugates to promote fusion with a negatively charged model membrane is determined. Their DNA delivery efficiency and cytotoxicity are assessed in vitro. Lipoplexes are administered in the mouse lung, and transgene expression Indeterminate inflammatory activity are measured. The results show that conjugation of 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) with C12E4 produces a carrier that can efficiently deliver DNA to cells, with negligible ­associated toxicity. Fusogenicity of the conjugates shows good correlation with in vitro transfection efficiency and crucially depends on the length of the polyether moiety of the detergent. Finally, DOPC-C12E4 reveals highly potent for in vivo DNA delivery and favorably compares to GL67A, the current golden standard for gene delivery to the airway, opening the way for further promising developments.

磷脂干洗剂偶联物被提出来用作什么是基因递送的结合各种载体。制法了11种氧化物，并对其的性质实施了深入分析。检测偶联物推动与带负电荷量的模式化膜结合的能力素质。在身体之外评诂这些的DNA递送率和生殖细胞致癌性。在小鼠肺里追施Lipoplex，并侧量转dna表达出来不知道好的支原体感染灵活性。数据呈现，1,2-二油酰基-sn-甘油-3-磷酸胆碱（DOPC）与C12E4的偶联诞生了种各种载体，能有效性地将DNA递送往组织细胞，关联毒副作用能缺少不计较。偶联物的融入原性与体内转染率兼备较好的重要性性，如果在一定能力上决定于于洗洁剂聚醚组成部分的宽度。另外，DOPC-C12E4在身体内部DNA递送方便症状突出，与近年使用在气管表观遗传递送的金规格GL67A相信具有着其优势，为进一点有进展前途的进展奠定了发展。重要性高性价比：DOPE-PEG-CH2COOHDOPE-PEG-FADOPE-PEG-MalDOPE-PEG-NH2DOPE-PEG-SCDOPE-PEIDPPE-Cap-BiotinDPPE-Cap-FolateDPPE-ICGDPPE-mPEGDPPE-PEG2-NH-Mal上面的好文章的内容起源多种期刊论文或论文参考文献，若有侵犯知识产权请搞好关系公司册除！