文献：DOPC-Detergent Conjugates: Fusogenic Carriers for Improved In Vitro and In Vivo Gene Delivery

小说家：Philippe Pierrat, Anne Casset, Dimitri Kereselidze, Marie Lux, Françoise Pons, Luc Lebeau学术论文友链： 英文论文：Phospholipid-detergent conjugates are proposed as fusogenic carriers for gene delivery. Eleven compounds are prepared and their properties are investigated. The ability of the conjugates to promote fusion with a negatively charged model membrane is determined. Their DNA delivery efficiency and cytotoxicity are assessed in vitro. Lipoplexes are administered in the mouse lung, and transgene expression Indeterminate inflammatory activity are measured. The results show that conjugation of 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) with C12E4 produces a carrier that can efficiently deliver DNA to cells, with negligible ­associated toxicity. Fusogenicity of the conjugates shows good correlation with in vitro transfection efficiency and crucially depends on the length of the polyether moiety of the detergent. Finally, DOPC-C12E4 reveals highly potent for in vivo DNA delivery and favorably compares to GL67A, the current golden standard for gene delivery to the airway, opening the way for further promising developments.

磷脂干洗剂偶联物被提供 是基因组递送的协同形式。配制了11种单质，并对其概念开始了科研。判断偶联物促进会与带负自由电荷的对模型膜协同的功能。在身体测评鸟卵的DNA递送转化率和上皮细胞致毒。在小鼠肺上使用Lipoplex，并检测的转表观遗传表示不选定的宫颈炎症几丁质酶。数据揭示，1,2-二油酰基-sn-甘油-3-磷酸胆碱（DOPC）与C12E4的偶联生成半个种平台，能够 有用地将DNA递送来体细胞，相应的致癌性能够 删掉不计入。偶联物的融为一体原性与身体外转染速度包括顺畅的有关性，而且在非常大层度上在于于洗衣剂聚醚部门的间距。后面，DOPC-C12E4在人体内DNA递送问题具体表现好品质，与近几年用以气管遗传基因递送的金标准化GL67A较之包括优质，为进几步有非常好的经济发展走上了市政道路。关于安利：DOPE-PEG-CH2COOHDOPE-PEG-FADOPE-PEG-MalDOPE-PEG-NH2DOPE-PEG-SCDOPE-PEIDPPE-Cap-BiotinDPPE-Cap-FolateDPPE-ICGDPPE-mPEGDPPE-PEG2-NH-Mal这小文章东西因素多种期刊论文或文献资料，要是有知识产权侵权请去联系当我们删了！