文献：基于甘草酸修饰的DSPE-PEG-PEI纳米粒联合递送阿霉素和Bcl-2 siRNA的肝靶向联合*

链接：//www.frontiersin.org/journals/pharmacology/articles/10.3389/fphar.2019.00004/full

作者：Guixiang Tian，Ruiyan Pan&，Bo Zhang，Meihua Qu，LianBo Lian，Hong Jiang，Zhiqin Gao，Jingliang Wu

节选：

依托于奈米用量递送整体的联席辽法已趋势将成为种有发展趋势的手段，它整合了俩种或很多**工作机制。本实验分离纯化了由1,2-二硬脂酰-sn-甘油-3-磷酸工业乙醇胺-聚乙二醇-聚醚酰亚胺（DSPE-PEG-PEI）和甘草次酸呈现白色质酸（GA-HA）组合成的肝靶点奈米粒（GH-DPP），用作联席递送阿霉素（DOX）和Bcl-2 siRNA。测试了载药GH-DPP纳米技术级粒（siRNA/DOX/GH-DPP）的粒度布置、zeta电位差和状态。定性分析了其对HepG2组织系的组织系摄食和身体组织系致毒。在荷瘤小鼠中评价了siRNA/DOX/GH-DPP的体中动物布置和***效率。可是取决于，siRNA/DOX/GH-DPP纳米技术级粒呈近球型，对HepG2组织系表达出极量信任性的组织系致毒。与简单递送DOX或Bcl-2 siRNA的无甘草次酸共递送系统性（siRNA/DOX/DPP）和GH-DPP奈米粒差距，siRNA/DOX/GH-DPP奈米粒才能引导一些组织细胞凋亡，并表現出更好的**功效。GH-DPP奈米粒才能此外将肿瘤化疗药和siRNA递送往*地域，在***中出显出硕大的潜质。

译文：

Combination therapy based on nano-sized drug delivery system has been developed as a promising strategy by combining two or more anti-tumor mechanisms. Here, we prepared liver-targeted nanoparticles (GH-DPP) composed of 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-polyethylene glycol-polyetherimide (DSPE-PEG-PEI) with Glycyrrhetinic acid-modified hyaluronic acid (GA-HA) for co-delivery of doxorubicin (DOX) and Bcl-2 siRNA. Particles size, zeta potential and morphology were determined for the drug-loaded GH-DPP nanoparticles (siRNA/DOX/GH-DPP). Cellular uptake and in vitro cytotoxicity were analyzed against HepG2 cells. In vivo bio-distribution and anti-tumor therapeutic effects of siRNA/DOX/GH-DPP were evaluated in H22-bearing mice. The results showed that siRNA/DOX/GH-DPP nanoparticles were nearly spherical and showed dose-dependent cytotoxicity against HepG2 cells. Compared to Glycyrrhetinic acid-free co-delivery system (siRNA/DOX/DPP) and GH-DPP nanoparticles for delivery of DOX or Bcl-2 siRNA alone, siRNA/DOX/GH-DPP nanoparticles could induce more cellular apoptosis, and showed higher anti-tumor effect. Herein GH-DPP nanoparticles could simultaneously deliver both chemotherapy drugs and siRNA into the tumor region, exhibiting great potential in anti-tumor therapy.

上海pg电子娱乐游戏app 动物保证关联产品设备：Cy5-PEG-DPPEPeptides PEG PLADSPE-PEG-SP94iRGD-PEG-DSPEPTP-PEG-COOHAngiopep-2-PEG-N3DMPE-PEG-CY3cRGD-PEG-PLGA

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