医学文献：Ultrafast Diffusion of a Fluorescent Cholesterol Analog in Compartmentalized Plasma Membranes作家：Nao Hiramoto-Yamaki, Kenji A. K. Tanaka, Kenichi G. N. Suzuki, Koichiro M. Hirosawa, Manami S. H. Miyahara, Ziya Kalay, Koichiro Tanaka, Rinshi S. Kasai … See all authors 文章超链接： Cholesterol distribution and dynamics in the plasma membrane (PM) are poorly understood. The recent development of Bodipy488-conjugated cholesterol molecule (Bdp-Chol) allowed us to study cholesterol behavior in the PM, using single fluorescent-molecule imaging. Surprisingly, in the intact PM, Bdp-Chol diffused at the fastest rate ever found for any molecules in the PM, with a median diffusion coefficient (D) of 3.4 µm2/second, which was ∼10 times greater than that of non-raft phospholipid molecules (0.33 µm2/second), despite Bdp-Chol's probable association with raft domains. Furthermore, Bdp-Chol exhibited no sign of entrapment in time scales longer than 0.5 milliseconds. In the blebbed PM, where actin filaments were largely depleted, Bdp-Chol and Cy3-conjugated dioleoylphosphatidylethanolamine (Cy3-DOPE) diffused at comparable Ds (medians = 5.8 and 6.2 µm2/second, respectively), indicating that the actin-based membrane skeleton reduces the D of Bdp-Chol only by a factor of ∼2 from that in the blebbed PM, whereas it reduces the D of Cy3-DOPE by a factor of ∼20. These results are consistent with the previously proposed model, in which the PM is compartmentalized by the actin-based membrane-skeleton fence and its associated transmembrane picket proteins for the macroscopic diffusion of all of the membrane molecules, and suggest that the probability of Bdp-Chol passing through the compartment boundaries, once it enters the boundary, is ∼10× greater than that of Cy3-DOPE. Since the compartment sizes are greater than those of the putative raft domains, we conclude that raft domains coexist with membrane-skeleton-induced compartments and are contained within them.

Bodipy488共轭高密度脂蛋白升高团伙（Bdp-Chol）的发展方向使咱们还可以安全使用单荧光团伙成相设计PM中的高密度脂蛋白升高做法。中值传播公式（D）为3.4µm2/秒，比非筏磷脂原子（0.33µm2/秒）大10倍，纵然Bdp-Chul将会与筏结构的域相关的。再者，Bdp-Chol在可超过0.5的时间段大小上不存在表現出受困的的情况 毫秒。在泡状PM中，肌动球蛋白质丝很多用尽，Bdp-Chol和Cy3结合在一起的二油酰基磷脂酰甲醇胺（Cy3-DOPE）以相同的Ds（中四位数）散出 = 5.8 和6.2µm2/秒），表述由于肌动球蛋白质的膜骨架仅将Bdp-Chol的D调低了泡状PM的约2倍，而将Cy3-DOPE的D调低约20倍。此类数据与开始之前要求的建模一直，在该建模中，PM被能够 肌动蛋清的膜骨架防护栏举例涉及的跨膜尖桩蛋清分割，适用每个膜团伙的宏观角度蔓延，并显示Bdp-Chol一旦发现开始的疆界，能够 隔室的疆界的的几率比Cy3-DOPE一般10倍。因为隔室厚度不低于假定的筏域，我们的求出结语，筏域与膜骨架引发的隔室共处，并包涵在之中。

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