期刊论文：Ultrafast Diffusion of a Fluorescent Cholesterol Analog in Compartmentalized Plasma Membranes做者：Nao Hiramoto-Yamaki, Kenji A. K. Tanaka, Kenichi G. N. Suzuki, Koichiro M. Hirosawa, Manami S. H. Miyahara, Ziya Kalay, Koichiro Tanaka, Rinshi S. Kasai … See all authors 文献综述外链： Cholesterol distribution and dynamics in the plasma membrane (PM) are poorly understood. The recent development of Bodipy488-conjugated cholesterol molecule (Bdp-Chol) allowed us to study cholesterol behavior in the PM, using single fluorescent-molecule imaging. Surprisingly, in the intact PM, Bdp-Chol diffused at the fastest rate ever found for any molecules in the PM, with a median diffusion coefficient (D) of 3.4 µm2/second, which was ∼10 times greater than that of non-raft phospholipid molecules (0.33 µm2/second), despite Bdp-Chol's probable association with raft domains. Furthermore, Bdp-Chol exhibited no sign of entrapment in time scales longer than 0.5 milliseconds. In the blebbed PM, where actin filaments were largely depleted, Bdp-Chol and Cy3-conjugated dioleoylphosphatidylethanolamine (Cy3-DOPE) diffused at comparable Ds (medians = 5.8 and 6.2 µm2/second, respectively), indicating that the actin-based membrane skeleton reduces the D of Bdp-Chol only by a factor of ∼2 from that in the blebbed PM, whereas it reduces the D of Cy3-DOPE by a factor of ∼20. These results are consistent with the previously proposed model, in which the PM is compartmentalized by the actin-based membrane-skeleton fence and its associated transmembrane picket proteins for the macroscopic diffusion of all of the membrane molecules, and suggest that the probability of Bdp-Chol passing through the compartment boundaries, once it enters the boundary, is ∼10× greater than that of Cy3-DOPE. Since the compartment sizes are greater than those of the putative raft domains, we conclude that raft domains coexist with membrane-skeleton-induced compartments and are contained within them.

Bodipy488共轭低密度胆固醇升高碳原子（Bdp-Chol）的趋势使我们的就能用单荧光碳原子成相学习PM中的低密度胆固醇升高手段。中值吸附指数（D）为3.4µm2/秒，比非筏磷脂分子式（0.33µm2/十几秒）大10倍，纵然Bdp-Chul将会与筏形式域有观。再者，Bdp-Chol在已经超过0.5的精力绝对误差上无成绩出离奇死亡的征兆 毫秒。在泡状PM中，肌动蛋白酶酶丝过多吸引，Bdp-Chol和Cy3切合的二油酰基磷脂酰工业乙醇胺（Cy3-DOPE）以该是的Ds（中十位数）蔓延 = 5.8 和6.2µm2/秒），表达研究背景肌动蛋白酶酶的膜骨架仅将Bdp-Chol的D降底了泡状PM的约2倍，而将Cy3-DOPE的D降底约20倍。等等毕竟与的时候提出来的模式化统一，在该模式化中，PM被因为肌动血清的膜骨架栅栏门以及相关内容的跨膜尖桩血清分仓缝，用以整个膜原子核的宏观环境蔓延，并表达Bdp-Chol若是迈入边缘线，经过隔室边缘线的概率统计比Cy3-DOPE大致需要10倍。仍然隔室寸尺不小于假定的筏域，小编给出总结，筏域与膜骨架帮助的隔室混用，并带有在另外。

相关推荐：

Cy3-DOPECy3-DPPECy3-DSPECy3-PEG-DOPECy3-PEG-DPPECy5-DOPECy5-DPPECy5-DSPECy5-PEG-DOPE不低于散文方式原因特殊杂志或论文资料，假如商标侵权请找话题各位删除文件！