论文：体现了提高粘液参透性的酸奶外泌体能作于口服方式递送siRNA下载链接：//pubs.rsc.org/en/content/articlehtml/2020/bm/d0bm01497d作著：Matthew R. Warren a、 Chenzhen 张 a、 Armin Vedadghavami a、 Krister Bokvist b、 Pradeep K. Dhal c和 Ambika G. Bajpayee * ad节选：荧光共轭 DSPE-PEG-叠氮化物的制法。 的使用DSPE-PEG-叠氮化物（下面统称DPA）在 mExo 单单从从表面成型疏水嵌入表格 PEG 耐磨涂层。关键在于将 DPA 以 2 mg mL −1的盐渗透压析出在 DMSO 中，第二逐滴更改到 1× PBS 中并不息均匀攪拌，使 DPA 的终极盐渗透压为 0.02 mg mL −1（7 μM），因而制法水溶性聚氨酯储备量库稀硫酸。对于那些在拆迁中遇到 DPA 盐渗透压批量的调查（即在线测量 mExo 单单从从表面短路电流和锚维持性调查），DPA实现叠氮化物-DBCO 点选化学物质反應与荧光大部分 DBCO-Cy5 运用。将 DBCO-Cy5（DMSO 中盐渗透压为 1 mg mL −1）逐滴更改到水溶性聚氨酯 DPA 储备量库液中，不息均匀攪拌，使终极摩尔之比 1 [细下划线（1/6-em）]：[细下划线（1/6-em）]4。第二在温度下猛地摇摆该稀硫酸以有助于点选反應点选反應后，将含有DPA-Cy5和否则DBCO-Cy5的稀硫酸会直接更改到外泌体备样中通过嵌入表格。原文翻译：Preparation of fluorescent-conjugated DSPE-PEG-Azide. DSPE-PEG-Azide (hereby DPA) was utilized to enable a hydrophobically-inserted PEG coating on the mExo surface. An aqueous stock solution was prepared by first dissolving DPA in DMSO at 2 mg mL−1, followed by dropwise addition to 1× PBS with continuous stirring for a final DPA concentration of 0.02 mg mL−1 (7 μM). For studies involving quantification of DPA concentration (i.e. measurement of mExo surface loading and anchor stability studies), DPA was conjugated to the fluorescent moiety DBCO-Cy5 via the azide-DBCO click chemistry reaction. DBCO-Cy5 (1 mg mL−1 in DMSO) was added dropwise with continuous stirring to aqueous DPA stock for a 1[thin space (1/6-em)]:[thin space (1/6-em)]4 final molar ratio. This solution was then incubated for 6 h at room temperature with light shaking to facilitate the click reaction. After the click reaction, the solution containing DPA-Cy5 and excess DBCO-Cy5 was added directly to exosome samples for insertion.

宝鸡pg电子娱乐游戏app 生物工程供给各种相关产品设备：DSPE-PEG-Cy5DSPE-PEG-mAb 2C5（二硬脂酰基磷脂酰酒精胺-聚乙二醇-*靶向疗法核蛋白)DSPE-PEG-OPSSCy5-DSPEDSPE-PEG-TetrazineDSPE-PEG-DAAsp6-PEG-DSPEDSPE-PEG2000-NGRXpeptide-PEG-DSPE以上的原创文章內容原因分类期刊论文或论文，要是有侵犯著作权请联系起来.我全部删除！