学术论文：Extracellular Vesicle Crosslinkers Constructing Hydrogels with Stress-Relaxation and Bioactive Protein Modification写作者：Shi, Yanzhen Jing, Haowen Lu, Fanxuan Zhao, Mengying An, Shuiling Jin, Chang Gao, Yongdong Dai, Yinxin Zhu, Shuxu Yang, Songying Zhang, Xuesong Ye, Xiujun Cai, Yifan Wang文献资料微信链接：

Here, we demonstrate the potential for the sequential insertion of two different DSPE-PEG amphiphilic molecules into the EV membrane: DSPE-PEG-NHS for protein conjugation and DSPE-PEG-SH for hydrogel crosslinking (Figure 4a–c). Using bovine serum albumin (BSA) as a model protein, we show the BSA band shifts upward in the gel band, indicating an increase in molecular weight after conjugation with DSPE-PEG-NHS, verifying the successful preparation of DSPE-PEG-BSA (Figure 4b). We also show that EVs modified with both DSPE-PEG-SH and DSPE-PEG-BSA could be successfully crosslinked with 8-arm PEG-Nb through thiol-ene photopolymerization (Figure 4d). However, the storage modulus of the hydrogels significantly decreased after incorporating DSPE-PEG-BSA, suggesting that the protein may interfere with the EV crosslinking reaction. To further explore protein presentation, we used green fluorescent protein (GFP) as a model protein to visualize its distribution within the hydrogel. Fluorescent imaging revealed that GFP was evenly distributed throughout the EV-crosslinked hydrogels, confirming the successful encapsulation of GFP into the hydrogel network (Figure 4e,f).

我们大家动态展示了将多种有差异的DSPE-PEG两亲性原子核依次读取EV膜的能力：DSPE-PEG-NHS代替球蛋白偶联，DSPE-PEG-SH代替水凝胶的作用化学交联。适用牛血清廉蛋清（BSA）看做模式化蛋清，你们体现 BSA带在疑胶带中上移移动手机，是因为与DSPE-PEG-NHS整合后原子核量加大，效验了DSPE-PEG-BSA的好提纯。小编还反映出，用DSPE-PEG-SH和DSPE-PEG-BSA表达的EV行进行硫烯光整合成功的 地与8臂PEG-Nb热塑。只不过，在参入DSPE-PEG-BSA后，水妇科抑菌凝胶的储能技术模量为显著减轻，意味着该核苷酸酶质应该会要素EV交连反應。为了让进一部思考核苷酸酶质的显现出，我们都动用纯天然荧光核苷酸酶（GFP）算作模型工具核苷酸酶来数据可视化其在水妇科抑菌凝胶中的分散。荧光三维成像体现GFP粗糙分布图制作在EV化学交联水凝露中，声明GFP完成包封到水凝露网络数据中。各种相关举荐：246256-50-8，5(6)-羧基四甲基罗丹明几丁质酶酯IR808双几丁质酶酯，IR808-NHS罗丹明标志甲基丙烯酰化明胶 GelMAFITC conjugated GelMADOTA-三甘氨酸，DOTA-GGG2183440-59-5，二磺酸基Cy7-马来酰亚胺2230212-27-6，sulfo Cy5(Me) SE146368-14-1，Sulfo-Cy5-E 虎珀酰亚胺酯上文散文资源源头几大类学术期刊或文献综述，假如有侵权商标请连接小编移除！