论文参考文献：Extracellular Vesicle Crosslinkers Constructing Hydrogels with Stress-Relaxation and Bioactive Protein Modification小说作家：Shi, Yanzhen Jing, Haowen Lu, Fanxuan Zhao, Mengying An, Shuiling Jin, Chang Gao, Yongdong Dai, Yinxin Zhu, Shuxu Yang, Songying Zhang, Xuesong Ye, Xiujun Cai, Yifan Wang论文资料联接：

Here, we demonstrate the potential for the sequential insertion of two different DSPE-PEG amphiphilic molecules into the EV membrane: DSPE-PEG-NHS for protein conjugation and DSPE-PEG-SH for hydrogel crosslinking (Figure 4a–c). Using bovine serum albumin (BSA) as a model protein, we show the BSA band shifts upward in the gel band, indicating an increase in molecular weight after conjugation with DSPE-PEG-NHS, verifying the successful preparation of DSPE-PEG-BSA (Figure 4b). We also show that EVs modified with both DSPE-PEG-SH and DSPE-PEG-BSA could be successfully crosslinked with 8-arm PEG-Nb through thiol-ene photopolymerization (Figure 4d). However, the storage modulus of the hydrogels significantly decreased after incorporating DSPE-PEG-BSA, suggesting that the protein may interfere with the EV crosslinking reaction. To further explore protein presentation, we used green fluorescent protein (GFP) as a model protein to visualize its distribution within the hydrogel. Fluorescent imaging revealed that GFP was evenly distributed throughout the EV-crosslinked hydrogels, confirming the successful encapsulation of GFP into the hydrogel network (Figure 4e,f).

各位分享了将有两种有所差异的DSPE-PEG两亲性氧分子按顺序嵌入EV膜的能力：DSPE-PEG-NHS使用淀粉酶质偶联，DSPE-PEG-SH使用水妇科凝胶热塑。施用牛血清廉蛋清（BSA）做对模型蛋清，我们都提示 BSA带在疑胶带中向左中国移动，体现了与DSPE-PEG-NHS配合后碳原子量多，检验了DSPE-PEG-BSA的顺利完成制作。我们公司还揭示，用DSPE-PEG-SH和DSPE-PEG-BSA表达的EV需要顺利通过硫烯光汇聚获得成功地与8臂PEG-Nb交连。因此，在添加DSPE-PEG-BSA后，水疑胶的储热模量明显大幅度降低，表示该营养物质质机会会干扰信号EV热塑表现。因为进步找寻营养物质质的显示，我国在使用浅绿色荧光营养物质（GFP）用于模式化营养物质来交互其在水疑胶中的分布区。荧光显像显视GFP匀占比在EV热塑水抑菌凝胶的作用中，验证GFP获得成功包封到水抑菌凝胶的作用网路中。对应举荐：246256-50-8，5(6)-羧基四甲基罗丹明亲水性酯IR808双活力性酯，IR808-NHS罗丹明箭头甲基丙烯酰化明胶 GelMAFITC conjugated GelMADOTA-三甘氨酸，DOTA-GGG2183440-59-5，二磺酸基Cy7-马来酰亚胺2230212-27-6，sulfo Cy5(Me) SE146368-14-1，Sulfo-Cy5-E 蜜腊酰亚胺酯超过稿件的内容源于各种各样学术期刊或参考文献，此事侵犯商标权请链接我门误删！