DOPE-PEG-Mal的合成与纯化方法
学术论文：同時阻隔 CD47 和 PD-L1 可提高先天畸形性和融入性肝癌免疫系统症状各类神经细胞细胞因子挥发超链接：//www.thelancet.com/article/S2352-3964(19)30158-6/fulltext小编：舒 莲，谢锐 志，叶玉英，谢晓东，李淑慧，路玉生，李碧飞，程云龙，弗拉基米尔·卡塔纳耶夫，李嘉节选：MAL-PEG-DOPE的合成图片MAL-PEG-DOPE的生成对比很早以前公布的本文。按照EDC/NHS工艺将MAL-PEG-COOH的羧基与DOPE的胺基运用。关键手段详细：将30mg羧基绘制的PEG互溶二氯二氧化氮中，并与5mgEDC和4mgNHS混合法，高温下连续式搅拌设备2h。然而下载8mg DOPE（MAL-PEG-COOH∶DOPE=1∶1，摩尔比），惰性气体保证下发应過夜。将发应结果在翻转视频蒸发器器仪中太干至大通常二氯二氧化氮，然而下载冷乙腈中。未发应的DOPE在2414g下离心式10min，不互溶冷乙腈。上清液在翻转视频蒸发器器仪中太干为稀脂质。将膜用DD水多化。将发应结果置入透析袋（分子式量= 8 k Da）中，并转变至50 mL DD水液体中，高温下发应48小的时候，离心分离分散的EDC/NHS/MAL-PEG-COOH。进而结果DOPE-PEG-MAL继而用冻干机冷却。考虑到手机验证DOPE-PEG-MAL的运用，对原材料实现了核磁共振检查现象波谱讲解。Synthesis of MAL-PEG-DOPESynthesis of MAL-PEG-DOPE refers to previously published articles [22,23]. The conjugation of carboxyl groups of MAL-PEG-COOH to the amine groups of DOPE was accomplished using the EDC/NHS technique. The process was carried out as follows: 30 mg carboxyl-modified PEG was dissolved in dichloromethane and mixed with EDC (5 mg) and NHS (4 mg). The solution was stirred continuously for 2 h at room temperature. Subsequently, 8 mg DOPE (MAL-PEG-COOH: DOPE =1:1, molar ratio) was added, and the reaction proceeded overnight under nitrogen. The reaction product was dried out most dichloromethane in rotary evaporator and then added to cold acetonitrile. The unreacted DOPE was centrifuged at 2414g for 10 min which was insoluble in cold acetonitrile. And the supernatant was dried to thin lipid in rotary evaporator. The film was hydrated with DD water. The reaction product was enclosed in dialysis bag (MW = 8 k Da) and transferred into 50 mL of DD water solution to separate free EDC/ NHS/ MAL-PEG-COOH at room temperature for 48 h. The final product DOPE-PEG-MAL was subsequently freezed by lyophilizer. To confirm the DOPE-PEG-MAL conjugation, the samples were examined by nuclear magnetic resonance spectroscopy.
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