专著：BioMed Research International文献资料外部链接：//onlinelibrary.wiley.com/doi/full/10.1155/2014/129458内容提要：Postmodification of the liposome surface by amine-carboxyl conjugation (Figure 1(b)) was recently reported to overcome the rapid systemic clearance of D-phenylalanyl-L-prolyl-L-arginyl-chloromethyl ketone short peptide (PPACK), an antithrombin agent [30]. Palekar et al.  demonstrated that PPACK peptide is attached to the surface of preformed liposomes composed of EPC, DPPE, and DSPE-PEG2000-COOH (94 : 4 : 2 molar ratio) by applying standard amine-carboxyl coupling conditions for conjugating the N-terminus of the peptide to preformed carboxy-terminated DSPE-PEG-containing liposomes. The peptide was conjugated to the unilamellar liposomes and liposomes were purified by dialysis for four hours, which suggests high stability of the nanosystem. 

经由胺羧基偶联对脂质身体面面参与后表达，都可以战胜抗凝血酶酶剂D-苯丙氨酰-L-脯氨酰基-L-精氨酰氯甲基酮短肽（PPACK）的怏速混身去除。Palekar抓捕经由利用前提条件胺羧基偶联前提条件将肽的N端偶联到即时分解成的含羧基封web端DSPE-PEG的脂质体上，验证了PPACK肽粘接在由EPC、DPPE和DSPE-PEG2000-COOH（94:4:2摩尔比）分解成的即时分解成的脂质体的面上。肽与一层脂质体能够，脂质体经由透析41天纯化，这表达微米设计具备有很高的平衡性。相关联举荐：C18-PEGn-MaleimideC18-PEGn-N3 (N3: Azide)C18-PEGn-NH2 (NH2: Amine)C18-PEGn-NHSC18-PEGn-OHC18-PEGn-OPSSC18-PEGn-SH (SH: Thiol)mPEG-Cholesterol (mPEG-CLS)mPEG-CONH-C12mPEG-CONH-C16mPEG-CONH-C18以下原创文章相关内容特征各个期刊杂志或论文，如无侵犯商标权请认识我门刪除！