文献资料：Characterization of RGD-modified liposomes for multimodal molecular imaging ofαvβ3 integrin-expressing pancreatic cancer小说作品：Mitsuyoshi Yoshimoto, Takuya Hayakawa, Masayuki Yamaguchi, Sadaaki Kimura and Hirofumi Fujii论文外部链接：//jnm.snmjournals.org/content/57/supplement_2/1206.shortMethods Liposomes composed of 1,2-distearoyl-sn-glycero-3-phosphocholine (DSPC), cholesterol and N-(carbonyl-methoxypolyethyleneglycol 2000)-1,2-distearoyl-sn-glycero-3-phosphoethanolamine (mPEG-DSPE) were prepared by thin film hydration method. RGD-modified liposomes were synthesized by coupling c(RGDfK)-SH with maleimide-mPEG-DSPE. The amount of RGD-modification was regulated by changing ratios of maleimide-mPEG-DSPE to total mPEG-DSPE (50%: SH-RGD-liposome, 5%: H-RGD-liposome, 2.5%: M-RGD-liposome, and 1%: L-RGD-liposome). As reference liposomes without targeting ability, RKG-liposome and unmodified liposome (NT-liposome) were prepared. The particle size was adjusted to 100 nm. Binding affinities of RGD-modified liposomes to αvβ3 integrin were assessed as IC50s for 125I-echistatine binding to PANC-1 human pancreatic cancer cells with expression of αvβ3 integrin. 111In-deferoxamine and Fe-deferoxamine were encapsulated into the liposomes for radionuclide and MR studies, respectively. Biodistribution and MR imaging were carried out in a PANC-1 xenograft model.

的方法用到聚酰亚胺膜水合法性制法由1,2-二硬脂酰基-sn-甘油-3-磷酸胆碱（DSPC）、固醇和N-（羰基甲氧基聚乙二醇2000）-1,2-二硬脂酰基-sn-甘油-3-磷工业乙醇胺（mPEG-DSPE）包含的脂质体。经过将c（RGDfK）-SH与马来酰亚胺mPEG-DSPE偶联，合成图片了RGD呈现的脂质体。RGD呈现的量经过修改马来酰亚胺mPEG-DSPE与总mPEG-DSPE的比例图来调结（50%：SH-RGD脂质体，5%：H-RGD脂质体，2.5%：M-RGD-脂质体，1%：L-RGD-脂质体）。作就没有靶向治疗学习能力的分类脂质体，制法了RKG脂质体和未装饰的脂质体（NT脂质体）。将比表面积的调节至100nm。 111In-deferosamine和Fe-deferosaine区分包封在脂质体当中于蔓延性核素和MR分析。在PANC-1异种复制物绘图中对其进行了菌物布置和MR影像。相关建议：DSPE-PEG-SHDSPE-PEG-SilaneDSPE-PEG-SP2-AADSPE-PEG-SSDSPE-PEG-TCODSPE-PEG-TetrazineDSPE-PEG-TMSDSPE-PEG-TPPDSPE-PEG-Transferrin DSPE-PEG-VE, MW:2000DSPE-PEG-β-cyclodextrinDSPE-PEG-Ald之内短文网站内容來源以及论文期刊或期刊论文，假如有知识产权侵权请连接小编删除文件！