文章：用到光镊绘画和重构仿生设计囊泡系统跳转：//www.nature.com/articles/s41467-018-04282-w做者：吉多·博洛涅西马克·S·弗里丁阿里巴巴·萨利希-雷哈尼内森·E·巴洛，尼古拉斯·J·布鲁克斯，奥斯卡·塞斯尤瓦尔·埃拉尼节选：囊泡融和和囊泡微作用器只为备制系统化的金nm离子囊泡，我国1在包含的2 wt.% 16:0 生态学素帽聚乙稀 (Biotinyl Cap PE) 的0.5 M白砂糖硫酸铜氢氧化钠溶液中电铸POPC囊泡。当来源于荧光脂质时，其水平为1 wt.%。在钙黄绿素就稀释和血清质表述实验操作中，囊泡确认保湿乳液相移动（见本文）转变成，氢氧化钠溶液含量为0.5 M白砂糖/草莓糖密度计算均值。现在来，将150 nm链霉亲和素包被的金nm离子（Nanopartz，英国科罗拉多州；产品的C11-150-TS-50；2.5 mg ml −1）以1:9的比率加上到囊泡中，将原辅料涡流四十分钟的英文以力促紧密结合。VIM的转变成最简单的方法与以后是一样的，但外接硫酸铜氢氧化钠溶液中的NaCl水平为0.25 M。确认在VIM画面上自动对焦并释放150 mW（在阱处）或更高些耗油率的缴光来确保囊泡的溶合。只为防止出现偶然溶合，安全施用少于100 mW的缴光（在阱处）对囊泡开展三维空间调节（摄入表明 13）。我国还安全施用了Aurora-DSGnm离子（AuNP记号的脂质；1-2 nm；Avanti），当其以2 wt.%的氢氧化钠溶液含量内嵌膜中时，不易引发溶合。

Vesicle fusion and vesicle microreactorsTo prepare functionalised AuNP vesicles, we first electroformed POPC vesicles in 0.5 M sucrose solution with 2 wt.% 16:0 Biotinyl Cap PE. When fluorescent lipids were present, these were at 1 wt.%. In the calcein dilution and protein expression experiments, vesicles were formed via emulsion phase transfer (see above), with 0.5 M sucrose/glucose density gradient. Next, 150 nm streptavidin-coated AuNPs (Nanopartz, CO, USA; product C11-150-TS-50; 2.5 mg ml−1) were added to the vesicles 1:9, with the sample vortexed for 30 min to drive conjugation. VIMs were formed as before, but with 0.25 M NaCl in the external solution. Vesicles were fused by focusing and applying a laser of 150 mW (at trap) or greater on the VIM interface. Vesicles were manipulated in space using a <100 mW laser (at trap) to avoid unintentional fusion (Supplementary Note 13). We also used Aurora-DSG Nanoparticles (AuNP-labelled lipids; 1–2 nm; Avanti), which did not result in fusion when embedded in the membrane at 2 wt.%.石家庄pg电子娱乐游戏app 生物制品供给有关產品：DSPE-PEG-LysozymeStearic acid-PEG-COOHDSPE-PEG-PLGADSPE-PEG-rhuMAb HER2-Fab’or scFv（二硬脂酰基磷脂酰工业乙醇胺-聚乙二醇-*靶点蛋白酶)cRGD-PEG-N3DSPE-PEG-Trastuzumab（二硬脂酰基磷脂酰无水乙醇胺-聚乙二醇-*靶向疗法淀粉酶)DSPE-PEG13-TFPDSPE-PEG-LSLERFLRCWSDAPA（二硬脂酰基磷脂酰工业乙醇胺-聚乙二醇-*靶向治疗蛋白质)DSPE-PEG-CSSRTMHHC（二硬脂酰基磷脂酰乙酸乙酯胺-聚乙二醇-*靶向疗法核蛋白)R6H4-PEG-DSPE上文章的内容的内容的来源各样学术期刊或学术论文，见谅知识产权侵权请结合各位删掉！