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Bi(mPEG-S-S)-TPE胶束的制备与表征
发布时间:2025-07-14     作者:zyl   分享到:
学术论文:AIE-Featured Redox-Sensitive Micelles for Bioimaging and Efficient Anticancer Drug Delivery原作者:by Wei Zhao 1,†,Zixue Li 1,†,Na Liang 2,*,Jiyang Liu 1,Pengfei Yan 1 andShaoping Sun 论文参考文献跳转:The drug-loaded Bi(mPEG-S-S)-TPE micelles were obtained as follows. At first, the Bi(mPEG-S-S)-TPE dispersion in distilled water was prepared. Then, the PTX in the acetone solution was added. After being sonicated for 3 min (on for 3 s and off for 2 s), the mixture was dialyzed (MWCO of 3500 Da) against distilled water for 2 h and further filtrated with a 0.45 μm filter to obtain the drug-loaded micelles. The micelles could be further freeze-dried and stored as a white powder.XRD analysis was used to analyze the crystalline characteristic of PTX in the micelles (Geigerflex, Rigaku Co., Tokyo, Japan). Data were collected from 5° to 50° with a step-scan mode. The dynamic light-scattering method was employed to determine the particle size and zeta potential of PTX-loaded micelles (Zetasizer Nano-ZS90, Malvern Instruments, Malvern, UK). The drug loading (DL) and encapsulation efficiency (EE) were calculated using Equations (2) and (3), respectively.

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Bi(mPEG-S-S)-TPE胶束的分离纯化与研究方法载药的Bi(mPEG-S-S)-TPE胶束有以下几点赢得。一开始,备制了Bi(mPEG-S-S)-TPE在减压萃取水里面的的分离体。之后,放入二甲苯液体中的PTX。经途5分钟钟的超声清洗补救(启闭3秒,封2秒)后,将混合着物用减压萃取水透析(MWCO为3500 Da)2半小时,之后用0.45μm滤水器进一大步滤水,得到了载药胶束。胶束就可以进一大步微冻非常干燥,并且以小白咖啡豆的的形式储藏。XRD研究定性分析选用研究定性分析胶束中PTX的析出性能指标。选用阶跃扫面模式英文从5°到50°回收利用大数据。选用动态化光散射法测定方法PTX短路电流胶束的粒度和ζ电势差(Zetasizer Nano-ZS90,Malvern Instruments,德国Malvern)。主要用式子式(2)和(3)来计算口服药短路电流量(DL)和包封成功率(EE)。相关内容分享:Silane-PEG-BiotinLA-PEG-BiotinIA-PEG-BiotinBiotin-PEG-ACAN3-PEG-BiotinOPSS-PEG-BiotinBiotin-PEG-MalBiotin-PEG-SCMSH-PEG-BiotinBiotin-PEG-OHBiotin-PEG-NH2以上的文章标题的内容來源种类学术期刊或医学文献,如果发现侵犯著作权请练习你们册除!