期刊论文：Biotin-conjugated PEGylated porphyrin self-assembled nanoparticles co-targeting mitochondria and lysosomes for advanced chemo-photodynamic combination therapy†作家：Baskaran Purushothaman , Jinhyeok Choi , Solji Park , Jeongmin Lee , Annie Agnes Suganya Samson , Sera Hong and Joon Myong Song论文链接代码：  In this study, biotin-conjugated PEGylated photosensitizer (PS) self-assembled nanoparticles (meso-tetraphenylporphyrin (TPP)–PEG–biotin SANs) were prepared via a self-assembly process to serve as nanocarriers for chemo-drugs as well as PSs. Electron microscopy results reveal the spherical shape of the nanoparticles (NPs). In the NPs, conjugated biotin plays a key role in selective tumor targeting. In vitro cellular experiments revealed the rapid cellular uptake of the TPP–PEG–biotin conjugates by MCF-7 cells that overexpress the biotin receptor, and verified that the conjugates were much more effective PSs than TPPS used as control in the cytotoxicity test. Interestingly, subcellular localization studies showed that the conjugates and their self-assembled NPs were localized mainly in mitochondria and partially in lysosomes, whereas TPPS was localized only in lysosomes. With the exclusive localization in mitochondria, high-content cell based assay showed that the TPP–PEG–biotin SANs induced rapid mitochondrial membrane potential transition (MPT), leading to cellular apoptosis. The chemo-drug doxorubicin (DOX) was successfully encapsulated in the TPP–PEG–biotin SANs (DOX@TPP–PEG–biotin) and had synergistic effects with enhanced cytotoxicity after PDT action. 

在这种实验中，经由自拼装具体步骤分离纯化了海洋生态学素偶联的聚乙二醇化光敏剂（PS）自拼装納米级塑料颗粒（meso-四苯基卟啉（TPP）-PEG-海洋生态学素-SAN），成为化工类药物和PS的納米级形式。手机光学显微镜最后信息显示了nm水粒子（NP）的圆形。在NP中，共轭菌物素在选用性良性肿瘤靶向疗法中起着关键因素效应。离体内部进行实验体现了了适度表明菌物素多巴胺受体的MCF-7内部对TPP-PEG-菌物素偶联物的迅速的内部摄取量，并认证了偶联物用无限流量卡比用作内部致癌性实验设计剖析的TPPS更更好。亚组织位置确定研究探讨表达，偶联物下列不属于自折装NP具体位置确定优酷云粒体中，一部分位置确定在溶酶体中，而TPPS仅位置确定在溶酶体。高纯度组织检测工具表达，TPP-PEG-菌物素SANs仅优酷云粒体中位置确定，可诱惑线粒体膜电极电位飞速转成（MPT），会造成组织凋亡。放疗化疗中药阿霉素（DOX）成就 封口在TPP-PEG-生态学素SAN中(DOX@TPP-PEG-生态学素），并在PDT用后具备着开展上皮细胞毒副效果的信息化用。有关于举荐：ICG-PEG-AlkyneICG-PEG-BiotinICG-PEG-CHOICG-PEG-COOHICG-PEG-cRGDICG-PEG-FolateICG-PEG-MALICG-PEG-N3ICG-PEG-NH2ICG-PEG-NHSICG-PEG-OHICG-PEG-SH