期刊论文：Biotin-conjugated PEGylated porphyrin self-assembled nanoparticles co-targeting mitochondria and lysosomes for advanced chemo-photodynamic combination therapy†写作者：Baskaran Purushothaman , Jinhyeok Choi , Solji Park , Jeongmin Lee , Annie Agnes Suganya Samson , Sera Hong and Joon Myong Song文献资料的链接： 小结： The TPP–PEG–biotin chemical was synthesized by the acid–amine coupling reaction between biotin–PEG–COOH and TPP-amine. Briefly, 1 mmol of biotin–PEG–COOH, DMAP, EDCI and 3 ml of CH2Cl2 were added to a glass vial and the reaction mixture stirred for 3 h at 0 °C. Then 1 mmol of TPP-NH2 was added and the reaction mixture was stirred continuously for 36 h at room temperature under a N2 atmosphere. The unconjugated TPP-NH2 and the EDCI adduct formed were removed by column chromatography using silica gel. Finally, the pure TPP–PEG–biotin chemical was dried completely using a lyophilizer and characterized by spectroscopy methods. MALDI-TOF MS: m/z 4169.3 [M]+.

TPP-PEG-生物技术素的转化成可以实现海洋微生物技术学素-PEG-COOH与TPP胺彼此的酸胺偶联响应转化成了TPP-PEG-海洋微生物技术学素检查是否物。简在于之，将1 mmol海洋微生物技术学素-PEG-COOH、DMAP、EDCI和3 ml CH2Cl2引入破璃瓶中，响应混合型式物在0°C下混合型机3分钟。但是引入1mmol TPP-NH2，在温度传到N2气质下间断混合型机响应混合型式物36分钟。可以实现透明硅胶柱色谱法清掉未偶联的TPP-NH2和生成的EDCI加合物。后，动用冻干机将纯TPP-PEG-海洋微生物技术学素检查是否物已经干燥的，并可以实现光谱仪法实现定性分析。MALDI-TOF MS:m/z 4169.3[m]+。相关的分享：NHS-PEG-COOHACA-PEG-COOHFA-PEG-COOHLA-PEG-COOHIA-PEG-COOHAC-PEG-COOHAlKyne-PEG-COOHCOOH-PEG-EPOCOOH-PEG-MACOOH-PEG-N3CHO-PEG-COOHBiotin-PEG-COOHFmoc-NH-PEG-COOHBoc-NH-PEG-COOHMal-PEG-COOH之内本文玩法因素几大类学术期刊或文献资料，知悉侵权行为请取得在线留言的删除图片！