论文：Biotin-conjugated PEGylated porphyrin self-assembled nanoparticles co-targeting mitochondria and lysosomes for advanced chemo-photodynamic combination therapy†写作者：Baskaran Purushothaman , Jinhyeok Choi , Solji Park , Jeongmin Lee , Annie Agnes Suganya Samson , Sera Hong and Joon Myong Song专著微信链接：绪论：The NPs were prepared according to a previously published procedure.33 Briefly, 50 mg of TPP–PEG–biotin was dissolved in 5 ml of chloroform, dried under a flow of nitrogen gas, and dried further under vacuum to get a film layer. For nanoparticle synthesis, the TPP–PEG–biotin film layer was hydrated with 900 μl of PBS (pH 7.4) and 100 μl of DMSO. Then the solution mixture was sonicated for 15 min, and uniform sized self-assembled TPP–PEG–biotin NPs were obtained slowly. The NPs were collected by centrifugation at 15[thin space (1/6-em)]000 rpm using a TJ-25 centrifuge (Beckman Coulter). The characterization of TPP–PEG–biotin SANs was done by TEM and SEM. The stock was diluted with PBS (pH 7.4) and used for in vitro experiments.

TPP-PEG-生物制品素自装配纳米技术微粒的制法NP是跟据的时候发布的子程序制作的。将50 mg TPP–PEG–生物体素溶解度在5 ml氯仿中，在惰性气体流着缺水，再在真空系统下进十步缺水以赢得膜层。针对奈米再生颗粒取得，TPP-PEG-微生态学素膜层用900μl PBS（pH 7.4）和100μl DMSO水合。第二将溶剂纯净物物超声清洗加工处理14钟头，变缓取得一致面积的自拆卸TPP-PEG-微生态学素NP。食用TJ-25离心力式机（Beckman Coulter）以15[薄室内空间（1/6-em）]000rpm的的速度离心力式回收NP。按照智能散射智能光学高倍显微镜（TEM）和扫描拍摄智能光学高倍显微镜（SEM）对TPP-PEG-动物素-SAN去了定量分析。将原液用PBS（pH 7.4）配制，用作身体外实验操作。对应个性化推荐：SC-PEG-CH2CH2COOtBuSC-PEG-CLASC-PEG-DEPSC-PEG-MTASC-PEG-OLASC-PEG-PLA(2K)SC-PEG-TretinoinSH-PEG-SCSilane-PEG-SCSTA-PEG-SCTocopherol-PEG-SCTocopherol-PEG-SCMA-PEG-MA CAS No.: 25852-47-5SAA-PEG-SAAGAA-PEG-GAASA-PEG-SAGA-PEG-GA以下原创文章的内容渠道各个刊物或文献资料，假如知识产权侵权请找话题各位册除！