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TPP-PEG-生物素自组装纳米粒子的制备
发布时间:2025-07-15     作者:zyl   分享到:
资料:Biotin-conjugated PEGylated porphyrin self-assembled nanoparticles co-targeting mitochondria and lysosomes for advanced chemo-photodynamic combination therapy†小说作品:Baskaran Purushothaman , Jinhyeok Choi , Solji Park , Jeongmin Lee , Annie Agnes Suganya Samson , Sera Hong and Joon Myong Song医学文献的链接:小结:The NPs were prepared according to a previously published procedure.33 Briefly, 50 mg of TPP–PEG–biotin was dissolved in 5 ml of chloroform, dried under a flow of nitrogen gas, and dried further under vacuum to get a film layer. For nanoparticle synthesis, the TPP–PEG–biotin film layer was hydrated with 900 μl of PBS (pH 7.4) and 100 μl of DMSO. Then the solution mixture was sonicated for 15 min, and uniform sized self-assembled TPP–PEG–biotin NPs were obtained slowly. The NPs were collected by centrifugation at 15[thin space (1/6-em)]000 rpm using a TJ-25 centrifuge (Beckman Coulter). The characterization of TPP–PEG–biotin SANs was done by TEM and SEM. The stock was diluted with PBS (pH 7.4) and used for in vitro experiments.

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TPP-PEG-生态学素自装配纳米级颗粒的光催化原理NP是依照事先公布了的程序代码准备的。将50 mg TPP–PEG–怪物素融化在5 ml氯仿中,在离氮气滴下干燥的处理,后来在真空系统下进两步干燥的处理以荣获膜层。相对 nm物体刷出,TPP-PEG-菌物素膜层用900μl PBS(pH 7.4)和100μl DMSO水合。后来将稀硫酸混合式物超声清洗进行处理1两一分钟,变缓刷出不均规格的自组装流水线TPP-PEG-菌物素NP。应用TJ-25抽滤机(Beckman Coulter)以15[薄前景(1/6-em)]000rpm的访问速度抽滤自身NP。确认散发出网络为了满足网络时代发展的需求,光学显微镜(TEM)和扫描仪扫描网络为了满足网络时代发展的需求,光学显微镜(SEM)对TPP-PEG-生物技术素-SAN参与了表现。将原液用PBS(pH 7.4)就稀释,于身体之外调查。各种相关网友推荐:SC-PEG-CH2CH2COOtBuSC-PEG-CLASC-PEG-DEPSC-PEG-MTASC-PEG-OLASC-PEG-PLA(2K)SC-PEG-TretinoinSH-PEG-SCSilane-PEG-SCSTA-PEG-SCTocopherol-PEG-SCTocopherol-PEG-SCMA-PEG-MA CAS No.: 25852-47-5SAA-PEG-SAAGAA-PEG-GAASA-PEG-SAGA-PEG-GA这文章标题网站内容从何而来各大学术期刊或学术论文,如不侵犯商标权请关系各位清空!