论文资料：Evaluation of the physicochemical properties and the biocompatibility of polyethylene glycol-conjugated gold nanoparticles: A formulation strategy for siRNA delivery小编：Kamil Rahme a b c 1, Jianfeng Guo d 1, Justin D. Holmes a b, Caitriona M. O’Driscoll期刊论文联接：前言：Recently, the potential of gold nanoparticles (AuNPs) for transporting drugs, proteins and genetic materials has been demonstrated. Previously, our laboratory synthesised positively charged, surfactant-free AuNPs in water by the reduction of gold (III) chloride (AuCl3) using hydroxylamine hydrochloride (NH2OH·HCl) in the presence of l-cysteine methyl ester hydrochloride (HSCH2CH(NH2)COOCH3·HCl) as a capping agent. These AuNPs, which achieve higher cell viability in comparison to cetyl trimethyl ammonium bromide (CTAB, a surfactant)-capped counterparts, have demonstrated potential for siRNA delivery. However, it is well known that systemic administration of cationic delivery systems without biological stablising moieties causes non-specific binding with negatively charged serum proteins, resulting in particle aggregation and opsonisation. Consequently, highly stable AuNPs capped with l-cysteine methyl ester hydrochloride conjugated to poly(ethylene glycol) (PEG) were synthesised in this study. PEGylation enhanced the biocompatibility of the AuNPs by reducing toxicity in a range of cell types, by inhibiting interaction with serum proteins thus avoiding aggregation, and, by providing protection against degradation by nucleases. Moreover, these PEGylated AuNPs formed nanoparticles (NPs) with siRNA (which was first compacted with protamine), and had a diameter within the nanoscale range (∼250 nm) and a near neutral surface charge (∼10 mV). In the future a bifunctional PEG chain on the AuNPs (i.e., SH-PEG-NH2, SH-PEG-COOH) will be used to facilitate conjugation of a targeting ligand to enhance cell specific uptake.

金nm粒子束（AuNP）在装运药品、淀粉酶质和基因类物质各方面的竟争力能够得到了印证。十年前，公司的研究室在l-半胱氨酸甲酯硝酸盐（HSCH2CH（NH2）COOCH3·HCl）看做封端剂的有着下，应用硝酸羟胺（NH2OH·HCl）恢复氯化金（III）（AuCl3），在一般的水都人工带正带电粒子、无外面渗透性剂的AuNP。与十五烷基三级甲等基溴化铵（CTAB，一些从表面可溶性剂）封中端分属物优于，以上AuNP兼具更大的神经细胞存留率，早已经单位证明了siRNA递送的潜能。既使，沒有生态学可靠的部分的阳铝离子递送整体的全身上下给药会诱发与带负正电荷的血清球蛋白的非特异形构建，为了诱发颗料密集和条理。于是，本的研究制成了用与聚乙二醇（PEG）共轭的l-半胱氨酸甲酯稀盐酸盐封端高强度可靠的AuNP。聚乙二醇化用降低了一产品系列组织业务类型的毒素、可以抑制与血清血清的完美用途最终得以处理聚在一起，相应出具处理核酸酶生物学降解的守护，增加了AuNP的生物学相融性。与此同时，一些聚乙二醇化的AuNP与siRNA（前提是用鱼精血清回填）建成奈米科粒（NP），直经在奈米级范围之内内（约250 nm），界面电荷量介于普通（约10 mV）。的前景，AuNP上的双功能键PEG链（即SH-PEG-NH2、SH-PEG-COOH）将采用利于靶向疗法配体的偶联，以加强細胞特女性朋友摄取量。相应推荐英文：Biotin-PEG-FABiotin-PEG-NHSAlkyne-PEG-BiotinSilane-PEG-BiotinLA-PEG-BiotinIA-PEG-BiotinBiotin-PEG-ACAN3-PEG-BiotinOPSS-PEG-BiotinBiotin-PEG-MalBiotin-PEG-SCMSH-PEG-BiotinBiotin-PEG-OH及以上的文章内部的来源分类刊物或专著，如无商标侵权请搞好关系当我们删掉！