文献综述：Tumor angiogenesis targeting and imaging using gold nanoparticle probe with directly conjugated cyclic NGR†诗人：Minghao Wu‡ ORCID logoa, Yanyan Zhang‡a, Ying Zhanga, Mingjie Wub, Menglin Wua, Hongyi Wua, Lin Caoa, Liang Lia, Xue Li*a and Xuening Zhang*a专著联接：//pubs.rsc.org/en/content/articlehtml/2018/ra/c7ra10155d内容提要：Preparation of FITC coupled GNPs–PEG@cNGR and GNPs–PEG.To prepare FITC coupled GNPs–PEG@cNGR and GNPs–PEG, 1 mg of the GNPs–PEG@cNGR probe, previously synthesized, was dispersed into 1 mL crosslinking reaction solution containing 7.56 mg Na2CO3, 1.06 mg NaHCO3, and 7.36 mg NaCl, sonicated for 2 min, and stored at 4 °C for 10 min. Subsequently, 0.3 mg FITC in 300 μL of DMSO was poured into the above solution under gentle and continuous stirring and incubated in the dark for 8 h at 4 °C. Next, 20 μL NH4Cl solution (5 M) was poured into the above solution under gentle and continuous stirring to terminate the above reaction and then incubated in the dark for 2 h at 4 °C. The final GNPs–PEG@cNGR@FITC was retrieved by ultrafiltration with a Millipore centrifugal filter unit (Amicon Ultra-0.5; MWCO 100[thin space (1/6-em)]000), washed three times, and resuspended in 0.5 mL of PBS (pH 7.4).In addition, FITC-conjugated GNPs–PEG (GNPs–PEG@FITC) was also prepared as a control. Briefly, the preparation was similar to the fabrication of GNPs–PEG@cNGR, but 220 μL SH–cNGR (1 mg mL−1) was replaced by 15 μL 2-aminoethanethiol (20 mM).

需要准备FITC合体的GNP——PEG@cNGR和GNP-PEG，1毫克GNP-PEGPEG@cNGR将开始之前自动合成的检测器分散性到具有7.56mg Na2CO3、1.06mg NaHCO3和7.36mg NaCl的1mL交连反响水溶液中，mri除理2一分钟的英文，在4°C下会自动储存10一分钟的英文。现在来，在一个清新陆续式混合下，将0.3mg FITC易溶于300μL DMSO中到入给出氢氧化钠盐饱和溶液中，在4℃下闭光孵育8分钟。现在来，在一个清新陆续式混合下将20μL NH4Cl氢氧化钠盐饱和溶液（5M）到入给出氢氧化钠盐饱和溶液中以中断给出发应，并且在4°C放到黑背地里孵育2分钟。不可能的GNPPEG@cNGR@使用的Millipore抽滤滤过器单位（Amicon Ultra-0.5；MWCO 100[薄空間（1/6-em）]000）通过反渗透装置，收废FITC，干洗俩次，重在新透明桌面在0.5 mL PBS（pH 7.4）中。再者，FITC偶联的GNP-PEG（GNP-PEG）PEG@FITC)也预备作照表。简来说 之，制取期间类试于GNP的生产制造——PEG@cNGR，但220μL SH-cNGR（1 mg mL-1）被15μL 2-氨基乙硫醇（20 mM）所改变。有关的介绍：Biotin-PEG-PFPDiethoxylsilane-PEG-BiotinMonoethoxylsilane-PEG-BiotinBiotin-PEG-TretinoinNTA-PEG-BiotinBiotin-PEG-SSBiotin-PEG-SGBiotin-PEG-HZBiotin-PEG-SVABiotin-PEG-ACBiotin-PEG-CHODBCO-PEG-BiotinBiotin-PEG-FA以上的小文章内部来历各种各样杂志或论文，请谅解抄袭请找大家删了！