DSPE-PEG-PDP改善金外层脂质体破损道德行为及tLBM产生逻辑钻研联结：//pubs.acs.org/doi/abs/10.1021/la300127m创作者：王曦马修·M·辛德尔王思文雷吉娜·拉根*引言：在水抗震液标准下，根据分子力显微镜观察 (AFM) 科学理论研究了脂质体（由特大型编织成单层囊泡组成的）与底材漆层之前的物理吸引力，实验设计其在金漆层安装驱动囊泡脱落和束搏脂质双层玻璃膜 (tLBM) 变成的使用。将 1,2-二硬脂酰-sn-甘油-3-磷酸甲醇胺-N-聚乙二醇-2000 -N- [3-(2-吡啶基二硫代)丙酸酯] (DSPE-PEG-PDP) 加上到 1-棕榈酰-2-油酰-sn-甘油-3-磷酸胆碱 (POPC) 囊泡中，以有利于促进确认金-硫醇盐键变成的互为使用。在有参透压和无参透压标准下，AFM 测试探头测试探头促进的以至于金漆层囊泡脱落的力被量化分析为 DSPE-PEG-PDP 成分的函数值。带来带有 2.5、5 和 10 mol % DSPE-PEG-PDP 的囊泡脱落需要备考的临介点力分离约为 1.1、0.8 和 0.5 nN。来说带有 2.5 mol % DSPE-PEG-PDP 的囊泡，tLBM 变成需要备考的临介点力从 1.1 nN（无参透压）减少为 0.6 nN（5 mM CaCl 2带来的参透压）。多达 5 nN 的力是不以至于纯 POPC 囊泡在金上变成 LBM。DSPE-PEG-PDP 仿佛来说在金漆层锚定和扭曲囊泡比较重要。这一项科学理论研究作品展示了如果根据基本交叉性脂质来的调节囊泡-漆层互为使用，并体现了了囊泡-底物互为使用在囊泡脱落中的使用。AbstractAtomic force microscopy (AFM) studies under aqueous buffer probed the role of chemical affinity between liposomes, consisting of large unilamellar vesicles, and substrate surfaces in driving vesicle rupture and tethered lipid bilayer membrane (tLBM) formation on Au surfaces. 1,2-Distearoyl-sn-glycero-3-phosphoethanolamine-N-poly(ethylene glycol)-2000-N-[3-(2-pyridyldithio) propionate] (DSPE-PEG-PDP) was added to 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) vesicles to promote interactions via Au–thiolate bond formation. Forces induced by an AFM tip leading to vesicle rupture on Au were quantified as a function of DSPE-PEG-PDP composition with and without osmotic pressure. The critical forces needed to initiate rupture of vesicles with 2.5, 5, and 10 mol % DSPE-PEG-PDP are approximately 1.1, 0.8, and 0.5 nN, respectively. The critical force needed for tLBM formation decreases from 1.1 nN (without osmotic pressure) to 0.6 nN (with an osmotic pressure due to 5 mM of CaCl2) for vesicles having 2.5 mol % DSPE-PEG-PDP. Forces as high as 5 nN did not lead to LBM formation from pure POPC vesicles on Au. DSPE-PEG-PDP appears to be important to anchor and deform vesicles on Au surfaces. This study demonstrates how functional lipids can be used to tune vesicle–surface interactions and elucidates the role of vesicle–substrate interactions in vesicle rupture.

山东pg电子娱乐游戏app 生态学展示对应设备：DSPE-PEG-acidDSPE-PEG4-acidDSPE-PEG-AldDSPE-PEG-Amine，1069-79-0DSPE-PEG-azideDSPE-PEG5-azideDSPE-PEG-BiotinDSPE-PEG-Boronate及以上新闻稿件信息从何而来各种各样期刊杂志或资料，比如侵权行为请取得联系公司误删！