学术论文：Liver-Targeted Combination Therapy Basing on Glycyrrhizic Acid-Modified DSPE-PEG-PEI Nanoparticles for Co-delivery of Doxorubicin and Bcl-2 siRNA做者：Guixiang Tian&#x;Guixiang Tian1†Ruiyan Pan&#x;Ruiyan Pan2†Bo Zhang&#x;Bo Zhang2†Meihua QuMeihua Qu2Bo LianBo Lian1Hong JiangHong Jiang1Zhiqin Gao*Zhiqin Gao1*Jingliang Wu*Jingliang Wu1*文献综述超链接：

Synthesis of DSPE-PEG-PEI ConjugatesBi-functional DSPE-PEG-NHS was used to conjugate with PEI via the primary amine reactive NHS ester moiety at weakly basic pH, thus avoiding the conjugation and crosslinking of the maleimide groups to the amine functions of PEI, which occurs at higher pH (pH > 8). The structure of DSPE-PEG-NHS, PEI and resulting DSPE-PEG-PEI copolymer were verified by 1H NMR. The peaks of PEG (3.6 ppm, -CH2O-), DSPE (1.0–1.5 ppm, -CH2-) and PEI (2.5–3.0 ppm, CH2-N) were confirmed. The1H-NMR spectrum of DSPE-PEG-PEI in D2O exhibited characteristic peaks at 2.5–3.0 ppm (peaks of PEI), 3.6 ppm (peaks of PEG) and 1.0–1.5 ppm (peaks of DSPE), indicating that PEI was successfully introduced to the DSPE-PEG-NHS molecular.

DSPE-PEG-PEI共轭物的制成双官能DSPE-PEG-NHS使用于在弱含碱pH下实现伯胺反馈性NHS酯环节与PEI偶联，最后逃避了马来酰亚胺基团与PEI胺官能团的偶联和热塑，这在较高pH值（pH>8）签发生。能够1H NMR确保了DSPE-PEG-NHS、PEI和所述DSPE-PEG-PEI共聚物的结构设计。PEG（3.6ppm，-CH2O-）、DSPE（1.0-1.5ppm，-CH2-）和PEI（2.5-3.0ppm，CH2-N）的峰已被确保。D2O中DSPE-PEG-PEI的1H NMR光谱图在2.5-3.0 ppm（PEI峰）、3.6 ppm（PEG峰）和1.0-1.5 ppm（DSPE峰）处显视出特证峰，表面PEI已经变成功转化DSPE-PEG-NHS碳原子。涉及到推建：DSPE-PEG-MethyltetrazineDSPE-PEG-MTXDSPE-PEG-nano goldDSPE-PEG-NBDSPE-PEG-NBDDSPE-PEG-NPCDSPE-PEG-NTA-NIDSPE-PEG-PCLDSPE-PEG-PDPDSPE-PEG-PEIDSPE-PEG-PLA上述优秀文章玩法来自特殊杂志期刊或期刊论文，烦请抄袭请练习我国误删！