文献：Au Nanocage Functionalized with Ultra-small Fe3O4 Nanoparticles for Targeting T1–T2Dual MRI and CT Imaging of Tumor
作者:Guannan Wang, Wei Gao, Xuanjun Zhang & Xifan Mei
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摘要：
The targeting ability of F-AuNC@Fe3O4 to folate receptor-overexpressed cancer cells was studied using A549 cells as an example. To more directly display the targeting capabilities of the F-AuNC@Fe3O4, the fluorescence Cy5-PEG-SH was selected to label the nanoparticls. In order to give clear fluorescence imaging and retain the ability of bio-functionalization, firstly, the mixture of COOH-PEG-SH and fluorescence Cy5-PEG-SH with 9:1 ratio was used to functionalize the surface of AuNC@Fe3O4 and then fluorescent AuNC@Fe3O4 was conjugated with targeting molecular folic acid to form the fluorescent F-AuNC@Fe3O4 for targeting cancer cell. As a control group, the fluorescent AuNC@Fe3O4 without surface folic acid also was used, which showed similar physical properties to those of fluorescent F-AuNC@Fe3O4. Figure 4a,b row show the confocal images of A549 cells after incubation with fluorescent F-AuNC@Fe3O4 and AuNC@Fe3O4 suspensions at a concentration of 0.5 mg/mL for one hour, respectively. The fluorescence image shown in Fig. 4a(i) clearly shows the successful internalization of the fluorescent F-AuNC@Fe3O4 into A549 cells. The much brighter red emission observed in Fig. 4a(i) as compared to that in Fig. 4b(i) (A549 cell incubation with fluorescent AuNC@Fe3O4) indicates that more F-AuNC@Fe3O4 can be internalized into A549 cells via folate receptor-mediated endocytosis and the F-AuNC@Fe3O4 have more capability for cancer targeting recognition. These results confirm the F-AuNC@Fe3O4 can be effectively targeted to the folate receptor expressed cancer cells.
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