mal-PEG-COOH的合成及其在功能聚合物修饰中的应用研究
医学文献:聚乙二醇-聚乙稀亚胺电动机扭矩超顺磁纳米级Fe3O4的镶嵌以及表观遗传转染软件编辑:姚琴,谢柏臻,裴一花专著网页链接: 前言:A water-soluble graft copolymer polyethylene glycol grafted branched polyethylene imine (mPEG-g-PEI) was obtained by modifying polyethylene imine (PEI) with monomethyl ether polyethylene glycol (mPEG-OH), and polyethylene glycol polyethylene imine loaded with superparamagnetic iron oxide (PEG-PEI-SPIO) was prepared by ion exchange method. Then, α - carboxyl - ω - maleimide polyethylene glycol (mal-PEG-COOH) and multifunctional SPIO loaded antibody polyethylene glycol polyethylene imine (Ab PEG PEI-SPIO) were synthesized. The composite of PEG-PEI-SPIO and plasmid DNA (pDNA) had a particle size of about 105 nm. In vitro experiments showed that T cells could specifically absorb... The Ab-PEG-PEI loaded SPIO targeting group showed good performance in gene transfection experiments, and real-time non-invasive observation can be performed using magnetic resonance imaging during the gene transfection process