论文资料：Nano-encapsulated tanshinone IIA in PLGA-PEG-COOH inhibits apoptosis and inflammation in cerebral ischemia/reperfusion injury小编：Xin Zhang , Xutong Zhu , Lifa Huang , Zupeng Chen , Yuchen Wang EMAIL logo , Yajun Liu , Ruihan Pan and Ling L期刊论文链接代码：英文论文：Tanshinone IIA has a potential therapeutic effect on cerebral ischemia/reperfusion injury (CIRI). In this study, tanshinone IIA was encapsulated in poly(lactic-co-glycolic acid)-block-poly (ethylene glycol)-carboxylic acid (PLGA-PEG-COOH) nanoparticles, and its therapeutic efficacy on CIRI was investigated. Morphology and dynamic light scattering analyses were performed to identify and optimize nano-formulations. A drug release test was conducted using the dialysis method. The cytotoxic effect of tanshinone IIA on human neuroblastoma cells (SH-SY5Y) and brain endothelial capillary cells (hCMEC/D3) was measured using the MTT assay. The protective effect of PLGA-PEG-COOH-encapsulated tanshinone IIA against CIRI was evaluated in oxygen and glucose deprivation/reoxygenation-induced SH-SY5Y/IR cells and middle cerebral artery occlusion (MCAO) rats. Results showed that PLGA-PEG-COOH-encapsulated tanshinone IIA promoted viability and inhibited apoptosis of SH-SY5Y/IR cells (P < 0.01). Moreover, PLGA-PEG-COOH-encapsulated tanshinone IIA facilitated the invasion of SH-SY5Y/IR cells and repressed inflammation in MCAO rats (P < 0.01). Noteworthy, PLGA-PEG-COOH-encapsulated tanshinone IIA combined with angiopep-2 peptide presented a better inhibitory effect on CIRI than tanshinone IIA alone (P < 0.01). Angiopep-2 peptide contributes to traversing blood–brain barrier by recognizing lipoprotein-related protein expressed in the brain capillary endothelial cells. In conclusion, PLGA-PEG-COOH-encapsulated tanshinone IIA plus angiopep-2 peptide holds promising therapeutic potential toward CIRI.

本论述将丹参酮IIA 包装在聚乳酸-羟基乙酸嵌段聚乙二醇-羧酸（PLGA-PEG-COOH）nm级颗粒状中，并论述其对CIRI的的治疗成效。实现型态学和静态光散射分享以鉴定结论和整合nm级中药制剂。操作透析法实现抗癫痫药物释放出来检测。选用MTT法检验丹参酮IIA对人神经系统母体神经元核瘤体神经元核（SH-SY5Y）和脑内羊皮毛一体细动静脉体神经元核（hCMEC/D3）的体神经元核毒副效应效应。在脑缺氧和红提糖放弃/复氧诱导性的SH-SY5Y/IR体神经元核和前脑中动脉血管闭塞（MCAO）大鼠中估评了PLGA-PEG-COOH包围的丹参酮IIA对CIRI的保护好效应。效果揭示，PLGA-PEG-COOH包丹参酮IIA可利于SH-SY5Y/IR血上皮细胞的自愈并促使其凋亡（P<0.01）。还有就是，PLGA-PEG-COOH包的丹参酮IIA利于了SH-SY5Y/IR血上皮细胞的入侵，并促使了MCAO大鼠（P<0.01）。引起考虑的是，PLGA-PEG-COOH 包装的丹参酮IIA与静脉肽-2肽联手采用对CIRI的克拍摄用远远高于简单采用丹参酮IIA（P<0.01）。Angiopep-2肽实现甄别脑毛细管静脉内皮細胞中形容的脂血清各种相关血清，有益于穿梭血脑防御系统。总的来说，PLGA-PEG-COOH包装的丹参酮IIA和动静脉肽-2肽对CIRI兼备好的进行治疗能力。重要性个性化推荐：CY5-PEG-FACy5-PEG-Hyd-DSPECy5-PEG-MalCY5-PEG-N3CY5-PEG-NH2Cy5-PEG-OHCY5-PEG-PCL(2K)Cy5-PEG-SCCY5-PEG-SHmPEG-PEI-CY5Cy7.5-PEG-AACY7.5-PEG-BiotinCY7.5-PEG-DBCOCY7.5-PEG-DSPECY7.5-PEG-FA

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