论文：Endocytic Mechanisms of Graphene Oxide Nanosheets in Osteoblasts, Hepatocytes and Macrophages小编：Javier Linares†M. Concepción Matesanz†Mercedes Vila‡§⊥M. José Feito†Gil Gonçalves⊥María Vallet-Reg퇧Paula A. A. P. Marques⊥M. Teresa Portolés文献综述联结： 内容提要：Nano-graphene oxide (GO) has attracted great interest in nanomedicine due to its own intrinsic properties and its possible biomedical applications such as drug delivery, tissue engineering and hyperthermia cancer therapy. However, the toxicity of GO nanosheets is not yet well-known and it is necessary to understand its entry mechanisms into mammalian cells in order to avoid cell damage and human toxicity. In the present study, the cellular uptake of pegylated GO nanosheets of ca. 100 nm labeled with fluorescein isothiocyanate (FITC-PEG-GOs) has been evaluated in the presence of eight inhibitors (colchicine, wortmannin, amiloride, cytochalasin B, cytochalasin D, genistein, phenylarsine oxide and chlorpromazine) that specifically affect different endocytosis mechanisms. Three cell types were chosen for this study: human Saos-2 osteoblasts, human HepG2 hepatocytes and murine RAW-264.7 macrophages. The results show that different mechanisms take part in FITC-PEG-GOs uptake, depending on the characteristics of each cell type. However, macropinocytosis seems to be a general internalization process in the three cell lines analyzed. Besides macropinocytosis, FITC-PEG-GOs can enter through pathways dependent on microtubules in Saos-2 osteoblasts, and through clathrin-dependent mechanisms in HepG2 hepatocytes and RAW-264.7 macrophages. HepG2 cells can also phagocytize FITC-PEG-GOs. These findings help to understand the interactions at the interface of GO nanosheets and mammalian cells and must be considered in further studies focused on their use for biomedical applications.

納米腐蚀苯（GO）由其一直有的成分基本在口服药推送、安排项目和热疗等微生物医疗行业领域的不确定适用，给予了很多人对納米医疗的很大程度上需求。其实，GO納米片的致毒尚不熟知清楚，有需要熟知其进乳期两栖动物内部的原则，以防范内部损害和身体致毒。在本的研究中，在8种仰药物（秋月仙素、渥曼宁、阿米洛利、组织变松下垂素B、组织变松下垂蛋白质D、纺织染料木素、苯胂被非金属氧化物和氯丙嗪）的存在的下，评估报告了用异硫氰酸荧光素（FITC-PEG-GO）标识的约100nm的聚乙二醇化GO纳米级片的组织摄入，这样的仰药物特定影向各个的内吞新机制。本研究探讨采用了多种神经元型：人Saos-2成骨神经元、人HepG2肝神经元和小鼠RAW-264.7巨噬神经元。结论说明，要根据每一种人体内部类别的优点，区别的原则体验了FITC-PEG-GOs的摄食。所以，在所探讨的六种人体内部系中，大小粒人体内部变多也许是一个个普通的内化方式。用来巨噬人体内部目的外，FITC-PEG-GOs还就能够经过Saos-2成骨人体内部中信任微管的手段渗入，并经过HepG2肝人体内部和RAW-264.7巨噬人体内部中的网格蛋清信任原则渗入。HepG2癌细胞核也就能够主宰FITC-PEG-GOs。这个显示能助认识GO微米片和喂母乳两栖动物癌细胞核界面显示上的能够 的作用，在进步的研究其在怪物医疗APP中的APP时可以对其进行选择。相关联个性化推荐：FITC-PEG-DMGFITC-PEG-FAFITC-PEG-BiotinOH-PEG-FAMNH2-PEG-FAMNTA-PEG-FITCFAM-PEG-N3FITC-PEG-ACFITC-PEG-ACAFITC-PEG-AlkyneFITC-PEG-AzithromycinFITC-PEG-CHOFITC-PEG-DTPAFITC-PEG-Estrogen以上相关内容优秀文章相关内容来源于各样中文核心期刊或期刊论文，如遇侵犯肖像权请建立联系让我们全部删除！