医学文献：Endocytic Mechanisms of Graphene Oxide Nanosheets in Osteoblasts, Hepatocytes and Macrophages原作者：Javier Linares†M. Concepción Matesanz†Mercedes Vila‡§⊥M. José Feito†Gil Gonçalves⊥María Vallet-Reg퇧Paula A. A. P. Marques⊥M. Teresa Portolés文章的链接： 提要：Nano-graphene oxide (GO) has attracted great interest in nanomedicine due to its own intrinsic properties and its possible biomedical applications such as drug delivery, tissue engineering and hyperthermia cancer therapy. However, the toxicity of GO nanosheets is not yet well-known and it is necessary to understand its entry mechanisms into mammalian cells in order to avoid cell damage and human toxicity. In the present study, the cellular uptake of pegylated GO nanosheets of ca. 100 nm labeled with fluorescein isothiocyanate (FITC-PEG-GOs) has been evaluated in the presence of eight inhibitors (colchicine, wortmannin, amiloride, cytochalasin B, cytochalasin D, genistein, phenylarsine oxide and chlorpromazine) that specifically affect different endocytosis mechanisms. Three cell types were chosen for this study: human Saos-2 osteoblasts, human HepG2 hepatocytes and murine RAW-264.7 macrophages. The results show that different mechanisms take part in FITC-PEG-GOs uptake, depending on the characteristics of each cell type. However, macropinocytosis seems to be a general internalization process in the three cell lines analyzed. Besides macropinocytosis, FITC-PEG-GOs can enter through pathways dependent on microtubules in Saos-2 osteoblasts, and through clathrin-dependent mechanisms in HepG2 hepatocytes and RAW-264.7 macrophages. HepG2 cells can also phagocytize FITC-PEG-GOs. These findings help to understand the interactions at the interface of GO nanosheets and mammalian cells and must be considered in further studies focused on their use for biomedical applications.

奈米级氧化的苯（GO）伴随其本身的性能举例说明在肿瘤药物气流输送、团体工程建筑和热疗等怪物分子生物学区域的因素应运，造成了消费者对奈米级分子生物学的很大的兴趣。那么，GO奈米级片的毒素尚不清除，有需要熟知其迈入喂母乳各种动物上皮细胞核的原则，以逃避上皮细胞核挤压伤和人体内毒素。在本论述中，在几种能够抑溶液剂（秋月仙素、渥曼宁、阿米洛利、上皮上皮人体细胞变松下垂素B、上皮上皮人体细胞变松下垂球蛋白D、纺织染料木素、苯胂化合物物和氯丙嗪）的存在着下，评价了用异硫氰酸荧光素（FITC-PEG-GO）标出的约100nm的聚乙二醇化GO纳米级片的上皮上皮人体细胞摄入，他们能够抑溶液剂专程印象各种的内吞体制。本的研究首选了三类細胞内型：人Saos-2成骨細胞、人HepG2肝細胞和小鼠RAW-264.7巨噬細胞。没想到意味着，会按照每款上皮神经細胞分类的显著特点，各种的原则组织了FITC-PEG-GOs的摄食。既使，在所分折的3种上皮神经細胞系中，大颗料上皮神经細胞越来越多仍然也是个常见的内化具体步骤。不但巨噬上皮神经細胞做用外，FITC-PEG-GOs还就能够顺利顺利通过Saos-2成骨上皮神经細胞中根据微管的有效途径来到，并顺利顺利通过HepG2肝上皮神经細胞和RAW-264.7巨噬上皮神经細胞中的网格蛋白酶根据原则来到。HepG2神经上皮细胞也应该吞食FITC-PEG-GOs。一些发现了这会有利于看待GO微米片和哺乳期爬行动物神经上皮细胞画质上的双方用途，在进三步科研其在生态学中医药学使用中的使用时不得不给以决定。有关的安利：FITC-PEG-DMGFITC-PEG-FAFITC-PEG-BiotinOH-PEG-FAMNH2-PEG-FAMNTA-PEG-FITCFAM-PEG-N3FITC-PEG-ACFITC-PEG-ACAFITC-PEG-AlkyneFITC-PEG-AzithromycinFITC-PEG-CHOFITC-PEG-DTPAFITC-PEG-Estrogen上面的稿件内部种类种类杂志或论文资料，比如商标侵权请联络我国删除文件！