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SH-PEG-COOH介导的金纳米棒多功能表面修饰及细胞穿透肽偶联
发布时间:2025-07-23     作者:zyl   分享到:
文献资料:Surface Functionalized Gold Nanorods: Tracking and Observing Live Cell via Three Optical Signals小说作者:Qiu, Xiaozhong; Wang, Yaling; Cui, Yanyan; Sun, Zhipeng; Liu, Ru; Huang, Wenhua; Zhao, Feng; Zu, Yan; Yuan, Hui; Chang, Xueling; Gao, and Xueyun期刊论文的链接://www.ingentaconnect.com/contentone/asp/jnn/2012/00000012/00000009/art00004引言:Surface functionalized gold nanorods (GNRs) with three optical signals and the ability to track and observe live cells was investigated. The Cetyltrimethyl Ammonium Bromide (CTAB) was used as the shape-directing agent to develop GNRs with uniform size and aspect ratio. The as-synthesized GNRs can strongly enhance the Raman signal of the attached MGITC on the GNRs. Polyethylene glycol (SH-PEG-COOH) and SH-PEG-FITC were attached with GNRs through ligand exchange with CTAB. They can stabilize the GNRs, reduce the cytotoxicity and provide fluorescence signal. A cell-penetrating peptide was covalently conjugated with SH-PEG-COOH to assist the GNRs to enter living cell. After cultured with the MCF-7 cells, the surfaced functionalized Au nanorods can enter the MCF-7 cells and be tracking with enhanced MGITC Raman signal, FITC fluorescence, and Rayleigh scatter signal.

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调查了还具有三种类型光数据信息的的表面系统化金纳米技术棒(GNR)已经跟踪定位和观察分析活人体细胞的意识。的使用第十六烷基三级甲等基溴化铵(CTAB)作外形抓手剂,发掘出长宽比和纵行比均的GNR。生成的GNR可能强促进GNR上粘着的MGITC的拉曼数据信息。聚乙二醇(SH-PEG-COOH)和SH-PEG-FITC根据与CTAB的配体交互与GNR衔接。什么和什么可不稳GNR,减少神经元致癌性并保证荧光数据信息。神经元穿透力肽与SH-PEG-COOH共价融合,以帮助到GNR流入活神经元。与MCF-7神经元同食训练后,从表面官能化的Au奈米棒可流入MCF-7神经元,接成激发的MGITC拉曼数据信息、FITC荧光和瑞利散射数据信息来追踪。关于介绍:Palmitic acid-PEG-MalPalmitic acid-PEG-NH2Palmitic acid-PEG-SCRB-PEG-DSPESTA-PEG-CHOSTA-PEG-COOHSTA-PEG-FASTA-PEG-FITCSTA-PEG-MalSTA-PEG-NBDSTA-PEG-NH2往上文章标题项目起源特殊杂志期刊或资料,若有抄袭请联系起来小编册除!