论文参考文献：Preparation and Characterization of Folate-Targeted Fe3O4 Nanoparticle Codelivering Cisplatin and TFPI-2 Plasmid DNA for Nasopharyngeal Carcinoma Therapy小说作者：Juan Zhang, Huanhuan Weng, Xiangwan Miao, Quanming Li, Siqi Wang, Huifen Xie, Tao Liu, Minqiang Xie参考文献联接： 前言：A novel folate (FA) receptor-targeted superparamagnetic Fe3O4 nanoparticles (SPIONs) codelivering cisplatin (CDDP) and tissue factor pathway inhibitor-2 (TFPI-2) plasmid DNA (pDNA) was constructed. The core shell nanocomposites (FA-PEG-PEI@SPION-CDDP-TFPI-2) were composed of superparamagnetic Fe3O4 core that binds CDDP and TFPI-2 shell that combines with folate-polyethylene glycol-polyethyleneimine (FA-PEG-PEI) via electrostatic interaction. The shell containing FA-PEG-PEI and TFPI-2 plasmid was synthesized through amidation reaction and electrostatic adsorption and the core containing SPION-CDDP was modified by aldehyde sodium alginate. Proton nuclear magnetic resonance and Fourier transform infrared spectra on FA-PEG-PEI polymers showed characteristic peaks of various metabolites in corresponding frequency. Transmission electron microscopy image of FA-PEG-PEI@SPION-CDDP-TFPI-2 nanoparticles demonstrated a near-monodisperse spherical morphology, while dynamic light scattering studies indicated an intensity-average diameter of 149.5 nm. Zeta potential was 14.89 ± 1.83 mv and the final concentration of loaded CDDP was 100 ug/ml. Gel electrophoresis data showed that the nanocomposite would protect TFPI-2 pDNA from being digested by DNases. Compared with CNE-2 cells, the good targetability and better gene transfection efficiency (57.9%) were detected by Prussian blue iron stain and fluorescence analysis in HNE-1 cells in vitro. The results suggested the potential application of FA-PEG-PEI@SPION-CDDP-TFPI-2 as a multifunctional anticancer nanomedicine on targeting therapy for FR positive NPC.

营造一种最新科技叶酸片（FA）蛋白激酶靶向药物的超顺剩磁Fe3O4奈米再生颗粒（SPION），其可各自递送顺铂（CDDP）和安排指数公式路径治理和改善剂-2（TFPI-2）质粒DNA（pDNA）。核壳纳米级混合素材(FA-PEG-PEI@SPION-CDDP-TFPI-2)由融入起来CDDP的超顺永久磁铁Fe3O4核和能够除感应电能够 使用与孕妇叶酸聚乙二醇聚丁二烯亚胺（FA-PEG-PEI）融入起来的TFPI-2壳组建。能够酰胺化症状和除感应电树脂吸附结合了包含的FA-PEG-PEI和TFPI-2质粒的的外壳，同用水藻酸钠醛突显了包含的SPION-CDDP的核心内容。FA-PEG-PEI汇聚物的质子核磁嗡嗡声和傅里叶更换红外光谱仪彰显了相对应平率下各种各样的代谢转化物的症状峰。散射微透射电镜观察数字图像FA-PEG-PEI@SPION-CDDP-TFPI-2奈米a粒子的表现出几乎单不集中的球体姿态，而动向光散射研发表示其強度平均水平直经为149.5 nm.Zeta电势差为14.89±1.83 mv，负载电阻CDDP的决定性酸度为100 凝胶的作用电泳数值展示，奈米复合文件文件是可以保护好TFPI-2 pDNA不被受到DNA酶的消化系统。与CNE-2受损神经细胞想必，普鲁士蓝铁复染和荧光进行分析在HNE-1受损神经细胞中检则到优质的靶向疗法性和更好的的表观遗传转染吸收率（57.9%）。涉及到的引荐：PLGA(20K)-PEG-OHPCL(5K)-PEG-OHPLA(2K)-PEG-OHOH-PEG-PLA(3K)6-NO2DA-PEG-OHOH-PEG-AAOH-PEG-SCMALKENE-PEG-OH CAS No.: 27274-31-3OH-PEG-PI(38K)PLGA(5K)-PEG-OHOH-PEG-AA-C30FA-PEG-OH以内玩法玩法来历几大类刊物或专著，假如知识产权侵权请保持联系让我们删除文件！