专著：Preparation and Characterization of Folate-Targeted Fe3O4 Nanoparticle Codelivering Cisplatin and TFPI-2 Plasmid DNA for Nasopharyngeal Carcinoma Therapy诗人：Juan Zhang, Huanhuan Weng, Xiangwan Miao, Quanming Li, Siqi Wang, Huifen Xie, Tao Liu, Minqiang Xie参考文献网页链接： 绪论：A novel folate (FA) receptor-targeted superparamagnetic Fe3O4 nanoparticles (SPIONs) codelivering cisplatin (CDDP) and tissue factor pathway inhibitor-2 (TFPI-2) plasmid DNA (pDNA) was constructed. The core shell nanocomposites (FA-PEG-PEI@SPION-CDDP-TFPI-2) were composed of superparamagnetic Fe3O4 core that binds CDDP and TFPI-2 shell that combines with folate-polyethylene glycol-polyethyleneimine (FA-PEG-PEI) via electrostatic interaction. The shell containing FA-PEG-PEI and TFPI-2 plasmid was synthesized through amidation reaction and electrostatic adsorption and the core containing SPION-CDDP was modified by aldehyde sodium alginate. Proton nuclear magnetic resonance and Fourier transform infrared spectra on FA-PEG-PEI polymers showed characteristic peaks of various metabolites in corresponding frequency. Transmission electron microscopy image of FA-PEG-PEI@SPION-CDDP-TFPI-2 nanoparticles demonstrated a near-monodisperse spherical morphology, while dynamic light scattering studies indicated an intensity-average diameter of 149.5 nm. Zeta potential was 14.89 ± 1.83 mv and the final concentration of loaded CDDP was 100 ug/ml. Gel electrophoresis data showed that the nanocomposite would protect TFPI-2 pDNA from being digested by DNases. Compared with CNE-2 cells, the good targetability and better gene transfection efficiency (57.9%) were detected by Prussian blue iron stain and fluorescence analysis in HNE-1 cells in vitro. The results suggested the potential application of FA-PEG-PEI@SPION-CDDP-TFPI-2 as a multifunctional anticancer nanomedicine on targeting therapy for FR positive NPC.

营造打了个种最新型叶酸片（FA）蛋白激酶靶向疗法的超顺带磁Fe3O4纳米技术物体（SPION），其可互相递送顺铂（CDDP）和集体指数公式经过仰剂型-2（TFPI-2）质粒DNA（pDNA）。核壳奈米黏结建材(FA-PEG-PEI@SPION-CDDP-TFPI-2)由配合在一起CDDP的超顺永久磁铁Fe3O4核和凭借感应电感应间接功效与叶酸片聚乙二醇聚氯乙烯亚胺（FA-PEG-PEI）配合在一起的TFPI-2壳形成。凭借酰胺化发生反应和感应电感应物理吸附合成图片了具有FA-PEG-PEI和TFPI-2质粒的表壳，互用海带酸钠醛绘制了具有SPION-CDDP的主导。FA-PEG-PEI缩聚物的质子核磁共鸣和傅里叶调整红外光谱图提示了相对应频段下各种各样消化吸收物的特征英文峰。散射微电子光学显微镜图象FA-PEG-PEI@SPION-CDDP-TFPI-2奈米级塑料再生颗粒表达出苛求单吸附的圆形价值形式，而最新光散射科学研究说明其承载力一般直劲为149.5 nm.Zeta电势为14.89±1.83 mv，负债CDDP的以后溶度为100 凝露电泳数据信息显视，奈米级组合板材可保护措施TFPI-2 pDNA遭受DNA酶的化解。与CNE-2血血细胞比起，普鲁士蓝铁脱色和荧光浅析在HNE-1血血细胞中查测到正常的靶向治疗性和好些的dna转染的效率（57.9%）。各种相关建议：PLGA(20K)-PEG-OHPCL(5K)-PEG-OHPLA(2K)-PEG-OHOH-PEG-PLA(3K)6-NO2DA-PEG-OHOH-PEG-AAOH-PEG-SCMALKENE-PEG-OH CAS No.: 27274-31-3OH-PEG-PI(38K)PLGA(5K)-PEG-OHOH-PEG-AA-C30FA-PEG-OH以下小文章方式源头种类杂志或资料，如遇侵犯肖像权请关联人们去除！