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PLL-PEG-PLL构建的PIC胶束用于DNA递送
发布时间:2025-07-24     作者:kx   分享到:
专著:PLL-PEG-PLL的光催化原理基本在DNA打包封装中的运用连接://link.springer.com/article/10.1007/s11426-006-2006-9小说作家:杨子刚,周芬,袁建军,马立新,翟超&程士元节选:利用对PLL(Z)-PEG-PLL(Z)中氨基对其进行脱养护,生成了PLL-PEG-PLL。深入分析了PLL-PEG-PLL与DNA对其进行的聚正离子黏结物(PIC)胶束的本质。结论得出结论,PIC的层面是由PLL与DNA利用防静电互相功用对其进行的,PEG为PIC的塑料外壳,之所以PIC胶束在水相中其匀匀称,虽然在一般的中性的条件下也不是会积累,更具充分的可靠性,这在什么是基因*中更具根本寓意。的同时,结论得出结论PLL-PEG-PLL需要对DNA对其进行缩合,且PLL嵌段越长,缩合效用越大,从而DNA的*核酸酶可溶性也为显著改善。PIC呈圆形,厚度为10~35 nm,适于被上皮肿瘤细胞摄取量。离体转染研究得出结论,所快件的DNA需要在SF9上皮肿瘤细胞系中表达方法。

PLL-PEG-PLL

PLL-PEG-PLL was synthesized by deprotection of the amino group of PLL(Z)-PEG-PLL(Z). The properties of the polyion complex (PIC) micelles comprising of PLL-PEG-PLL and DNA were investigated. The results showed that the core of PIC was formed by PLL and DNA through electrostatic interactions, and PEG was shell of PIC, so PIC micelles in aqueous medium were homogenous with no precipitation even under the neutral condition, and PIC had the characteristic of stability, which were very important in gene therapy. At the same time, the results showed PLL-PEG-PLL could condense DNA, longer PLL blocks were generally more effective at condensation, and the nuclease resistance of DNA was increased remarkably. PIC was spherical with diameter of 10–35 nm, which could be easily taken up by cells. Transfection experiments in vitro showed that the DNA encapsulated could be expressed in the cell line SF9.绵阳pg电子娱乐游戏app 微生物给予相应车辆:PLL-g-PEG-BiotinPLL-g-PEG-N3PLL-PEGPLL-PEG-N3PLL-PEG-OPSSPoly(L-Aspartic acid)-PEGsPoly(L-Ornithine)-MPEG往上软文介绍由来各个期刊杂志或参考文献,如果侵犯商标权请连接咱们删除文件!