论文资料：MICRORHEOLOGICAL QUANTIFICATION OF CELL-MEDIATED REMODELING OF POLYMER-PEPTIDE HYDROGELS IN RESPONSE TO CHEMICAL CUES专著友链： 提要：The next chapter describes the development of a new technique to create hydrogels with tethered concentration gradients of polymers or proteins. This technique also enables us to measure the diffusion coefficient of solutes in hydrogels. Hydrogels with tethered concentration gradients of cell signaling molecules can be used to direct cell migration from the scaffold to the wound after implantation. A rectangular prism is the basis of our microfluidic device. Before using the microfluidic device to create hydrogels with a tethered concentration gradient of TNF-α, we make concentration gradients with a model polymer, Fluorescein poly(ethylene glycol)-thiol (FITC-PEG-SH), to validate the technique. We first make a calibration curve by measuring hydrogels with known concentrations of FITC-PEG-SH and measure their arbitrary brightness using a laser scanner. This calibration curve relates brightness with tethered molecule concentration. We form hydrogels with tethered concentration gradients of FITC-PEG-SH by allowing the molecule to diffuse for 6, 24 and 48 hours. Hydrogels with tethered concentration gradients of FITC-PEG-SH are placed on the laser scanner and spatial brightness is measured. The calibration curve is then used to back-calculate local concentrations every 25 micrometers along the hydrogel. Next, we curve-fit a solution of Fick’s second law of diffusion to calculate the effective diffusion coefficient of FITC-PEG-SH. In the FITC-PEG-SH experiments, we hypothesize that steady state diffusion is reached when a gradient forms for 24 hours. We use the same method to make concentration gradients of thiolated and dyed TNF-α tethered into hydrogels. 

该工艺能否手工制造科技工艺更具高原子化合物物或蛋清质桎梏氧含量等度方向的水妇科疑胶。该工艺还使大家才可以自动测量水妇科疑胶中溶质的传播指数。更具癌组织讯号原子系留氧含量等度方向的水妇科疑胶都可以于在移植后鼓励癌组织从固定架转至到患处。多边形三棱镜是大家微流体动力动力保护仪器的根本。在用微流体动力动力保护仪器手工制造科技工艺更具TNF-α桎梏氧含量等度方向的水妇科疑胶的时候，大家用模式高原子化合物物荧光素聚乙二醇硫醇（FITC-PEG-SH）加工氧含量等度方向，以查验该工艺。咱们第一个能够 检测已发现渗透压的FITC-PEG-SH水抑菌凝露的作用来画出标定线性拟合线条，并应用智能机械扫视器仪检测其任意尺寸对比度对比度。该标定线性拟合线条将对比度对比度与系留原子渗透压练习起來。咱们能够 让原子传播6、24和48个天，确立具FITC-PEG-SH束搏渗透压比率的水抑菌凝露的作用。将具FITC-PEG-SH束搏渗透压比率的水抑菌凝露的作用放入在智能机械扫视器仪上，并检测空间对比度对比度。随后应用标定线性拟合线条沿水抑菌凝露的作用每25微米换算反方向统计公式一天轮廓渗透压。收起来，咱们对菲克二、传播基本定律的解进行线性拟合线条线性拟合，以统计公式FITC-PEG-SH的有效的传播比率。在FITC-PEG-SS实验操作中，咱们选用当比率确立24个天时可达到准稳态传播。咱们应用不同的工艺使巯基化和上色的TNF-α的渗透压比率不变在水抑菌凝露的作用中。检测的比予测说法值快有一个數量级的很好粘附指数公式。在建设好几个水抑菌疑胶复制到品后，我国选用快速速的粘附是在TNF-α和水抑菌疑胶化学交联剂期间的消除静电留住。这办法都可以扩大或缩减，以提纯都具有系留浓度值因子的水抑菌疑胶，使用于体细胞递送，或检测的水抑菌疑胶中汇聚物或球脂肪酸的粘附指数公式。相关联高性价比：AA-PEG-AAAlkyne-PEG-SVAPLGA(2K)-PEG-SCDBCO-PEG-SVAGala-PEG-SCMGluc-PEG-SCMSCM-S-PEG-S-SCMSVA-PEG-SVASPA-PEG-SPASAS-PEG-SASGAS-PEG-GASSG-PEG-SG之上经典文章內容源各项中文核心期刊或论文，比如抄袭请连接自己删掉！