论文资料：MICRORHEOLOGICAL QUANTIFICATION OF CELL-MEDIATED REMODELING OF POLYMER-PEPTIDE HYDROGELS IN RESPONSE TO CHEMICAL CUES文献综述联接： 英文论文：The next chapter describes the development of a new technique to create hydrogels with tethered concentration gradients of polymers or proteins. This technique also enables us to measure the diffusion coefficient of solutes in hydrogels. Hydrogels with tethered concentration gradients of cell signaling molecules can be used to direct cell migration from the scaffold to the wound after implantation. A rectangular prism is the basis of our microfluidic device. Before using the microfluidic device to create hydrogels with a tethered concentration gradient of TNF-α, we make concentration gradients with a model polymer, Fluorescein poly(ethylene glycol)-thiol (FITC-PEG-SH), to validate the technique. We first make a calibration curve by measuring hydrogels with known concentrations of FITC-PEG-SH and measure their arbitrary brightness using a laser scanner. This calibration curve relates brightness with tethered molecule concentration. We form hydrogels with tethered concentration gradients of FITC-PEG-SH by allowing the molecule to diffuse for 6, 24 and 48 hours. Hydrogels with tethered concentration gradients of FITC-PEG-SH are placed on the laser scanner and spatial brightness is measured. The calibration curve is then used to back-calculate local concentrations every 25 micrometers along the hydrogel. Next, we curve-fit a solution of Fick’s second law of diffusion to calculate the effective diffusion coefficient of FITC-PEG-SH. In the FITC-PEG-SH experiments, we hypothesize that steady state diffusion is reached when a gradient forms for 24 hours. We use the same method to make concentration gradients of thiolated and dyed TNF-α tethered into hydrogels. 

该能力都可以加工制作业含有高原子核物或球营养素绳束调教盐氧含量均值方向的水抑菌凝露。该能力还使.我也能测试水抑菌凝露中溶质的粘附数值。含有血細胞预警原子核系留盐氧含量均值方向的水抑菌凝露也可以于在植入性后引导作用血細胞从卡子转移到伤疤。长方形反射镜是.我微气体设备的基本。在使用的微气体设备加工制作业含有TNF-α绳束调教盐氧含量均值方向的水抑菌凝露之后，.我用模式高原子核物荧光素聚乙二醇硫醇（FITC-PEG-SH）加工制作盐氧含量均值方向，以确认该能力。大家先是能够 在在线測量已知a有机废气质量酸度的FITC-PEG-SH水抑菌抑菌妇科抑菌抑菌凝胶来画出效准折线，并便用二氧化碳激光机器阅读仪在在线測量其其中任何光色彩饱和度。该效准折线将光色彩饱和度与系留团伙有机废气质量酸度连续来。大家能够 让团伙对外粘附转移作用6、24和两天英文，变成有FITC-PEG-SH束博调教有机废气质量酸度等度的水抑菌抑菌妇科抑菌抑菌凝胶。将有FITC-PEG-SH束博调教有机废气质量酸度等度的水抑菌抑菌妇科抑菌抑菌凝胶放上在二氧化碳激光机器阅读仪上，并在在线測量房间光色彩饱和度。而后便用效准折线沿水抑菌抑菌妇科抑菌抑菌凝胶每252um逆向算一些身体局部有机废气质量酸度。下面来，大家对菲克二是对外粘附转移作用推论的解对其进行折线线性拟合，以算FITC-PEG-SH的有效地对外粘附转移作用弹性系数。在FITC-PEG-SS实验室中，大家如果当等度变成24分钟英文时以达到准稳态对外粘附转移作用。大家便用重复的方式 使巯基化和印染的TNF-α的有机废气质量酸度等度调整在水抑菌抑菌妇科抑菌抑菌凝胶中。测试比預測认识论值快两个用户级的很好分散指数。在新建许多水凝露黏贴品后，我们公司假如速度快速的分散是基于TNF-α和水凝露热塑剂期间的静电能吸引女生。一种方式会放小或缩减，以配制极具系留氧化还原电位等度的水凝露，使用在神经细胞递送，或测试水凝露中缩聚物或蛋白酶质的分散指数。各种相关介绍：AA-PEG-AAAlkyne-PEG-SVAPLGA(2K)-PEG-SCDBCO-PEG-SVAGala-PEG-SCMGluc-PEG-SCMSCM-S-PEG-S-SCMSVA-PEG-SVASPA-PEG-SPASAS-PEG-SASGAS-PEG-GASSG-PEG-SG这些论文內容源种类期刊杂志或文章，见谅侵犯商标权请练习各位删去！