资料:Gutenberg-style printing of self-assembled metal nanoparticle Aarays: electrostatic nanoparticle immobilisation and DNA-mediated transfer小说作家： Zheng, Yuanhui文献资料友链：内容提要： HS–PEG–COOH was diluted in citrate solution while stirring at room temperature. An amount of DTT was added in the solution with molar ratio DTT/HS–PEG–COOH about 1/1. Here the DTT produces the free sulfhydryl bonds. The solution DTT–HS–PEG–COOH then was added into primary AuNPs solution while stirring at room temperature for about 15 min. The minimum amount of HS–PEG–COOH required to stabilize AuNPs was determined by the method similar to the BSA case. Two serial dilutions of the HS–PEG–COOH–AuNPs (Au@PEG) at pH=4 and pH=6.5 were prepared, varying the quantities of HS–PEG–COOH from 0 to 150 μg in 1 ml of AuNPs solution (μg  ml −1), and followed by UV-VIS spectrophotometric analysis. The minimum amount of HS–PEG–COOH necessary to coat AuNPs was deduced graphically from the concentration with which the absorbance at pH=4 becomes nearly constant. All of these solutions were measured by UV-Vis spectrophotometry. The size and shape of AuNPs were characterized by a transmission electron microscope (TEM).

在温度下掺和的并且，将HS-PEG-COOH摇匀在百香果酸盐溶剂中。在溶剂添成为必参考值的DTT，DTT/HS–PEG–COOH的摩尔比约为1/1。徘徊里，DTT产生了解离巯基键。第三将DTT-HS-PEG-COOH溶剂成为到全能AuNP溶剂中，并且在温度下掺和约1五几分钟 min。安稳AuNP需要的HS-PEG-COOH的是较为小的量是实现相仿于BSA的方法步骤确定好的。HS-PEG-COOH-AuNPs的两大类产品系列稀释溶解液(Au@PEG)在pH=4和pH=6.5的先决条件下，将HS-PEG-COOH的量从0变动到150 μg/1 ml金微米级粒状稀硫酸（μg  ml-1），但是做好UV-VIS分光光度分析一下。给出pH=4时吸光度可以说平稳的浓度值，以图文模式推证出涂覆AuNP需求的最低HS-PEG-COOH量。整个这一些稀硫酸都完成红外光谱-可見分光光度法做好了在线测量。完成散发出智能显微镜观察（TEM）对金微米级粒状的大小和的形状做好了定量分析。涉及介绍：CLS-PEG-SilaneCLS-PEG-FITCCLS-PEG-BiotinCLS-PEG-MalCLS-PEG-FACLS-PEG-SHCLS-PEG-NH2CLS-PEG-COOHCLS-PEG-SCmPEG-CLS8-ArmPEG-CLS4-ARMPEG-CLSCLS-PEG-CLS上面好文章文章来历常见刊物或论文，以免侵权商标请连续我移除！