参考文献：Preparation and Characterization of Folate-Targeted Fe3O4 Nanoparticle Codelivering Cisplatin and TFPI-2 Plasmid DNA for Nasopharyngeal Carcinoma Therapy原作者：Juan Zhang, Huanhuan Weng, Xiangwan Miao, Quanming Li, Siqi Wang, Huifen Xie, Tao Liu, Minqiang Xie期刊论文图片链接：//onlinelibrary.wiley.com/doi/full/10.1155/2017/2849801英文论文：1H NMR was determined to confirm the synthesis of FA-PEG-PEI (the spectrum and relevant chemical structural formula were shown in Figure 1). The characteristic peaks from 2.0 to 3.0 ppm (k, j) were attributed to -CH2CH2NH- group of PEI, the peaks at 3.66 ppm (h) were referred to -CH2CH2O- group of PEG, and the peaks from 6.5 to 9.0 ppm (a, b, c) were related to the H protons of benzene ring in FA [25]. The data suggested a successful combination of FA-PEG-PEI.The possible functional groups responsible for the synthesis of FA-PEG-PEI were further confirmed by FT-IR spectroscopy. Meanwhile, the FT-IR spectra of FA, NH2-PEG-OH, and NH2-PEG-COOH were also examined. The terminal hydroxyl groups of NH2-PEG-OH were of carboxylation and then combined with the amino groups of PEI via amidation reaction. As shown in Figure 2, the FT-IR spectra of terminal hydroxyl groups of (b) NH2-PEG-OH showed the presence of stretching vibration peak of -OH at 3440 cm−1, which shifted to a new stretching vibration peak of -C=O at 1629 cm−1 in (c) NH2-PEG-COOH after carboxylation. The FT-IR spectra of (d) FA-PEG-PEI displayed a broad peak at 3350–3250 cm−1, which refers to -NH2 and -NH- groups of PEI. The peaks at 1606–1460 cm−1 correspond to the benzene ring of FA. In addition, the peak at 1110 cm−1 is attributed to the symmetrical stretching vibration of -C-O-C- group in PEG, which declares the conjugation of FA-PEG-PEI.

法测定1H NMR以根本FA-PEG-PEI的组成。优点最高值在2.0到3.0之間 ppm（k，j）归因于PEI的-CH2CH2NH-基团，最高值在3.66 ppm（h）说的是PEG的-CH2CH2O-基团，峰在6.5至9.0之間 ppm（a，b，c）与FA中苯环的H质子有关系。信息得出结论，FA-PEG-PEI的组成是顺利完成的。FT-IR光谱图仪进这一步表明了也许 承担FA-PEG-PEI生成的官能团。同一，还研究分析了FA、NH2-PEG-OH和NH2-PEG-COOH的傅里叶调整红外光谱图仪。NH2-PEG-OH的后部羟基被羧化，那么能够 酰胺化影响与PEI的氨基构建。长为2一样，（b）NH2-PEG-OH后部羟基的FT-IR光谱仪仪显现，在3440处存在着-OH的伸收高频共振峰 cm-1，在1629处手机移动到新的延展高频共振峰-C=O 羧化后，在（c）NH2-PEG-COOH里添加入cm-1。（d）FA-PEG-PEI的FT-IR光谱仪仪在3350-3250处显现一个多个宽峰 cm-1说明PEI的-NH2和-NH-基团。1606年至1460年的基线 cm-1相当于于FA的苯环。除此以外，1110处的峰 cm-1归因于PEG中-C-O-C-基团的中心对称伸收高频共振，这声明了FA-PEG-PEI的共轭。一些引荐：HZ-PEG-OHPhthalimide-PEG-OHPLL-PEG-OHPLGA(10K)-PEG-OHSilane-PEG-OHOH-PEG-PAOH-PEG-SSOH-PEG-SGLA-PEG-OH以上信息信息信息来历各种各样杂志或参考文献，如不抄袭请联络企业删掉！