文献：Preparation and Characterization of Folate-Targeted Fe3O4 Nanoparticle Codelivering Cisplatin and TFPI-2 Plasmid DNA for Nasopharyngeal Carcinoma Therapy

作者：Juan Zhang, Huanhuan Weng, Xiangwan Miao, Quanming Li, Siqi Wang, Huifen Xie, Tao Liu, Minqiang Xie

文献链接：//onlinelibrary.wiley.com/doi/full/10.1155/2017/2849801

摘要：

A poly(lactic-co-glycolic acid)-poly(ethylene glycol)-carboxyl (PLGA-PEG-COOH) and gelatin-based microparticles (MPs) dual delivery system for release of bone morphogenetic protein-2 (BMP-2) and insulin-like growth factor-1 (IGF-1) is developed. The delivery system is characterized based on its morphology, loading capacity, encapsulation efficiency, and release kinetics. The effects of electron beam (EB) sterilization on BMP-2 and IGF-1-loaded MPs and their biological effects are examined. The synergistic effect of a controlled dual release of BMP-2 and IGF-1 on osteogenesis of human mesenchymal stem cells (MSCs) is evaluated. Encapsulation efficiency of growth factors into gelatin and PLGA-PEG-COOH MPs is in the range of 64.78% to 76.11%. E-beam sterilized growth factor delivery systems are effective in significantly promoting osteogenesis of MSCs, although E-beam sterilization decreases the bioactivity of growth factors in MPs by ≈22%. BMP-2 release behavior from gelatin MPs/PEG hydrogel shows a faster release (52.7%) than that of IGF-1 from the PLGA-PEG-COOH MPs/PEG hydrogel (27.3%). The results demonstrate that the gelatin and PLGA-PEG-COOH MPs-based delivery system can realize temporal release of therapeutic biomolecules by incorporating different growth factors into distinct microparticles. EB sterilization is an accessible method for sterilizing growth factors-loaded carriers, which can pave the way for implementing growth factor delivery in clinical applications.

开发技术好几回种广泛用于产生骨状态造成核蛋白-2（BMP-2）和胰岛素样生长的指数公式-1（IGF-1）的聚乳酸-聚乙二醇-羧基（PLGA-PEG-COOH）和明胶基水分子（MP）双递送设备。递送设备的的特点最为其状态、额定负载学习能力、包封成功率和产生扭矩学。研发了智能束（EB）灭菌处理对装载BMP-2和IGF-1的MP的功用以至于生物工程学因素。评估方法了BMP-2和IGF-1的受控双从施放对世间充质体细胞膜（MSCs）成骨的协同工作功用。

生长因子在明胶和PLGA-PEG-COOH MP中的包封效率在64.78%至76.11%之间。电子束灭菌的生长因子递送系统能有效显著促进MSCs的成骨，尽管电子束灭菌会使MP中生长因子的生物活性降低约22%。明胶MPs/PEG水凝胶的BMP-2释放行为比PLGA-PEG-COOH MPs/PEG凝胶的IGF-1释放行为（27.3%）更快（52.7%）。

結果呈现，来源于明胶和PLGA-PEG-COOH MP的递送系统性也可能完成将有差异 的产生要素加入适量有差异 的粒子志为实现目标制疗性生物学氧分子的已经移除。EB无菌处理就是种可能用在于对配载产生要素的形式去无菌处理的具体方法，也可能为在诊疗app中进行产生要素递送抹平高架道路。有关于选择：N3-PEG-SVAFA-PEG-N3N3-PEG-SSN3-PEG-SGPhosphoric acid-PEG-N3N3-PEG-PAA(1.8K)-DAN3-PEG-NHSN3-PEG-AlkeneACA-PEG-N3AC-PEG-N3AlKyne-PEG-N3COOH-PEG-N3MA-PEG-N3N3-PEG-EPON3-PEG-OH以下经典文章知识种类多种学术期刊或专著，予以图片侵权请结合自己刪除！